The genetic diversity of radish germplasm resources was analyzed with RAPD. A total of 109 distinct bands were detected with 12 informative RAPD primers among 56 radish accessions from different countries or regions. Of them, 72 bands(62.9%) were polymorphic. The average heterozygosity of RAPD loci was 0.289 among 56 accessions. All accessions were clustered into two groups and nine subgroups based on the RAPD data by the method of clustering analysis, and three groups and seven subgroups by the method of principal coordinates analysis. The results from these two methods were similar, but the result from the principal coordinate analysis was more informative.