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FtMYB1转录因子调控苦荞毛状根黄酮醇合成的机理研究
伍小方1,2,3, 高国应1,2,3, 左倩1,2, 赵辉2, 张凯旋2, 严明理1,3, 周美亮2
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(1湖南科技大学生命科学学院,湘潭 411201;2中国农业科学院作物科学研究所,北京 100081;3湖南科技大学经济作物遗传改良与综合利用湖南省重点实验室, 湘潭 411201)
摘要:
R2R3-MYB 转录因子在植物类黄酮合成中起重要的调控作用。本研究从川荞 1 号中克隆获得一个 R2R3-MYB 家族基因 FtMYB1 ,酵母转录激活分析显示其具有转录激活活性,激素处理下的表达模式分析显示, FtMYB1 基因能够被茉莉酸甲酯(MeJA)和脱落酸(ABA)诱导表达。在苦荞毛状根中过表达 FtMYB1 基因,结果表明转基因毛状根株系中总黄酮和芦丁含量显著低于野生型,且在黄酮醇合成途径下游分别编码黄酮醇合酶(FLS)和鼠李糖基转移酶(RT)的 FLS 和 RT1 基因表达量在转基因株系中显著降低,表明 FtMYB1 可能通过调控 FLS 和 RT1 的表达来抑制黄酮醇的生物合成。
关键词:  R2R3-MYB转录因子  黄酮醇  苦荞  毛状根
DOI:10.13430/j.cnki.jpgr.20200118001
投稿时间:2020-01-18修订日期:2020-02-13
基金项目:国家重点研发计划中国-欧盟国际合作重点项目(2017YFE0117600);国家自然基金面上项目(31871536)
Deciphering the Functional Basis of FtMYB1 Transcription Factor in Flavonol Biosynthesis of Tartary Buckwheat Hairy Root
WU Xiao-fang1,2,3, GAO Guo-ying1,2,3, ZUO Qian1,2, ZHAO Hui2, ZHANG Kai-xuan2, YAN Ming-li1,3, ZHOU Mei-liang2
(1College of Life Sciences,Hunan University of Science and Technology, Xiangtan 411201;2Institute of Crop Sciences,Chinese Academy of Agricultural Sciences, Beijing 100081;3Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, Hunan University of Science and Technology, Xiangtan 411201)
Abstract:
R2R3-MYB transcription factors are known to play important regulation role in the flavonoid biosynthesis of plants. In this study, a gene FtMYB1 belonging to the R2R3-MYB gene family was cloned from Tartary buckwheat cultivar ‘Chuanqiao No.1’.The FtMYB1 transcription factor was found to exhibit the transcriptional activation activity in yeast, and its expression of FtMYB1 gene was induced by Methyl jasmonate (MeJA) and abscisic acid (ABA) treatment. Overexpressing the FtMYB1 gene in Tartary buckwheat hairy root showed a significant reduction in contents of total flavonoid and rutin if compared with wild type. Furthermore,the expression of FLS and RT1 genes, which encode for flavonol synthetase (FLS) and rhamnosyltransferase (RT) in Tartary buckwheat, respectively, were significantly lower in the FtMYB1 overexpressing hairy roots than that of wild type. Taken together, our results suggested that FtMYB1 may function in inhibition of the flavonol biosynthesis by repressing the expression of FLS and RT1 genes.
Key words:  R2R3-MYB transcription factor  flavonol  Tartary buckwheat  hairy root

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