单性结实樱桃番茄种质材料研究与抗病新种质创制
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浙江省农业科学院蔬菜研究所

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国家重点研发计划(2017YFD0101902);浙江省农业新品种选育重大科技专项(2016C02051);国家产业化项目 (CARS-23-G44);国家重点研发计划(政府间合作)(2017YFE0114500)


Study on Germplasm Materials of Parthenocarpic Cherry Tomato and Creation of New Germplasm with Dual Resistance Against Tomoto Yellow Leaf Curl Virus Disease and Gray Leaf Spot Disease
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Institute of Vegetables, Zhejiang Academy of Agricultural Sciences

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    摘要:

    以5份单性结实樱桃番茄种质材料为研究对象,应用苗期人工接种和分子标记辅助技术鉴定了材料黄化曲叶病毒病 (TYLCVD)和灰叶斑病(GLSD)的抗病性。材料CP28为研究对象,构建遗传群体,分析了单性结实性状的遗传规律;以 及利用超薄石蜡切片技术观察在日温15°C/夜温5°C、日温25°C/夜温15°C等2种温度栽培处理下子房的发育过程;以常规的杂 交和分子标记辅助相结合的手段进行了抗TYLCVD和GLSD单性结实新种质的创制。结果发现:经鉴定,5份材料都不抗 TYLCVD,材料CP24和CR3表现出抗GLSD;通过对遗传群体内单性结实性状分离情况分析,得出材料CP28的单性结实性状 由隐性单基因控制的结论;通过石蜡切片可以看出,日温15°C/夜温5°C低温栽培条件下子房内几乎没有胚珠,日温25°C/夜温 15°C温度栽培条件下子房内有数量多少不等的胚珠,并且能正常发育;创制了3份兼抗TYLCVD和GLSD单性结实新种质,为 多抗性单性结实新品种的培育奠定了基础。

    Abstract:

    The resistance of five parthenocarpic cherry tomato materials against tomoto yellow leaf curl virus disease (TYLCVD) and gray leaf spot disease (GLSD) were identified using pathogen artificial inoculation and molecular markers. The genetic population was constructed using CP28, and the genetic law of parthenocarpy was studied. The ovary development process was observed at two different temperature environment (day/night temperature of 15°C/5°C and 25°C/15°C) using ultra thin paraffin section technology. The TYLCVD and GLSD parthenocarpic cherry tomato germplasm was constructed by regular hybridization and molecular markers. The results showed that no material was resistant to TYLCVD, but CP24, CR3 were resistant against GLSD. Based on the analysis of segregation of the genetic population, we concluded that the parthenocarpic trait of CP28 was controlled by recessive monogene. The paraffin sections showed no ovule in the ovary of tomato plants grown under low temperature (day/night temperature of 15°C/5°C), while some normal developed ovules were observed in tomato plants grown under normal temperature (day/night temperature of 25°C/15°C). Three new parthenocarpic germplasm resistant to both TYLCVD and GLSD were constructed, It laid a foundation for breeding of new cherry tomato varieties with parthenocarpy and multiple resistance.

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阮美颖,叶青静,程远,等.单性结实樱桃番茄种质材料研究与抗病新种质创制[J].植物遗传资源学报,2020,21(5):1148-1155.

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  • 收稿日期:2020-01-07
  • 最后修改日期:2020-03-30
  • 录用日期:2020-03-31
  • 在线发布日期: 2020-09-09
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