In order to effectively utilize the wheat germplasm introduced from abroad, high molecular weight glutenin subunits composition and quality properties were analyzed for 393 wheat accessions collected from CIMMYT, France, Pakistan and other foreign sources. The results showed that there were 23 types of subunits and 50 types of subunit combinations among the accessions with a high genetic variation and abundant subunits composition types. According to the subunits quality scores, the range of the introduced wheat germplasm was 4-10, with 7.98 as the average, with 74, 76 and 96 accessions (accounting for 62.6% of the total) scored 10, 9 and 8, and containing 8, 2 and 14 subunit combination types, respectively. Besides, 9 accessions were detected to contain new subunits and subunit combination types in this study: F14 (2*, 14*+15*/6*+16*, Null), F33 (2*, 14*+15*/6*+16*, Null), C119 (1, 7*+16*, 5+10), C120 (Null, 7*+16*, 5+10), C125 (2*, 7+8, 5’+10), C51 (2*, 7+9, 5’+10), O13 (2*, 7+16, 2+12) , O17 (2*, 7+16, 2+12) and P27 (2*, 7+18, 2+12). The introduced wheat germplasm on the whole are high in distribution frequency of good subunits, rich in subunit composition types, and may provide resources for utilization and quality improvement breeding.