On the basis of low-glucosinolate and low-erucic rapeseed (Brassica napus), stable high-oleic rapeseed is one of the most important breeding objectives. In this study, CRISPR/cas9 system was used to improve the variety XY15 selected by Hunan Agricultural University. BnaFAD2 and BnaFAE1 are two key genes for the conversion of oleic acid to unsaturated fatty acids and ultra-long-chain fatty acids. The CRISPR/Cas9 system was introduced into XY15 by using highly efficient rapeseed transformation technology. The results of target gene cloning and sequencing showed that none of the seven T0 transgenic plants obtained were edited. However, gene editing was found and detected in T1 plants. The total gene editing efficiency of BnaFAD2.A05, BnaFAD2.C05, BnaFAE1.A08 and BnaFAE1.C03 were 38.89%, 27.78%, 22.22% and 30.56%, respectively. Among the T2 plants, 5 plants and 4 target genes were all mutated, and the relative oleic acid content of the mutants Cas9-1-12-4, Cas9-1-12-5, Cas9-2-7-11, Cas9-5-5-3 and Cas9-6-6-1 without foreign genes was 68.69%, 80.16%, 75.82%, 75.97% and 74.37%, respectively, which were significantly higher than that of the wild type XY15 (62.04%).