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  • 张盼,赵璇,耿玉璐,等.甘蓝型油菜 - 白芥渐渗系材料农艺性状和种子品质分析[J].植物遗传资源学报,2020,21(1):63-73.    [点击复制]
  • ZHANG Pan,ZHAO Xuan,GENG Yu-lu,et al.Agronomic Traits and Qualitative Characters Analysis of Brassica napus-Sinapis alba Introgression Lines[J].植物遗传资源学报,2020,21(1):63-73.   [点击复制]
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甘蓝型油菜 - 白芥渐渗系材料农艺性状和种子品质分析
张盼, 赵璇, 耿玉璐, 郑宇茜, 蒋金金, 吴健, 方玉洁, 王幼平
0
(扬州大学生物科学与技术学院,扬州 225009)
摘要:
远缘杂交能够有效拓宽物种的遗传背景,产生丰富的遗传变异。前期研究中利用体细胞融合技术获得了甘蓝型油 菜扬油 6 号(Y6)与白芥的属间杂种,将杂种与 Y6 连续回交和自交得到了一系列稳定遗传的渐渗系株系。本研究以 62 个 渐渗系株系为材料,对这些株系进行 14 个农艺性状的考察,并通过相关性分析、主成分分析、回归分析和聚类分析等方法,探 究甘蓝型油菜单株产量与农艺性状之间的关系及影响单株产量的主要因素。结果显示,甘蓝型油菜 - 白芥渐渗系株系的农艺 性状存在较大差异;单株产量与全株角果数、有效分枝数和每角粒数等性状呈极显著正相关,而与分枝点高度呈极显著负相 关。主成分分析的结果表明,4 个主成分可以概括 14 个农艺性状的大部分信息,这些主成分主要与角果数目、株高、角果长度 和千粒重等性状指标相关。回归分析结果表明,千粒重、每角粒数、二次有效分枝数、株高和全株角果数对单株产量具有重要 影响。综合各项指标,筛选出一些具有优异农艺性状的材料,如 L8 等株系角果数目明显增多;L3、L7 等株系分枝数目明显增 多;L42、L49 等株系千粒重明显增加;L3、L8 等株系单株产量明显提高。利用近红外光谱仪分析渐渗系材料种子品质性状, 发现硫代葡萄糖甘、芥酸、亚麻酸等品质性状存在较大变异,可用于选育具有优良品质性状的材料。本研究表明远缘杂交对于 甘蓝型油菜种质资源创新、提高油菜产量提供了一条有效途径,研究结果为利用这些种质资源进行育种实践提供了理论依据。
关键词:  甘蓝型油菜  白芥  体细胞杂交  农艺性状  单株产量  种子品质
DOI:10.13430/j.cnki.jpgr.20191126004
投稿时间:2019-11-26修订日期:2019-11-26
基金项目:国家重点研发计划(2016YFD0102000);国家重点基础研究发展计划(2015CB150201);国家自然科学基金(31872874);大学生科创基金(x20180675)
Agronomic Traits and Qualitative Characters Analysis of Brassica napus-Sinapis alba Introgression Lines
ZHANG Pan, ZHAO Xuan, GENG Yu-lu, ZHENG Yu-qian, JIANG Jin-jin, WU Jian, FANG Yu-jie, WANG You-ping
(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009)
Abstract:
Distant hybridization is recognized as an effective strategy enriching the genetic abundance of the cultivated species. Taking use of the intergeneric hybrids of Brasssica napus(cultivar Yangyou 6,referred Y6) and Sinapis alba L. with somatic cell fusion as well as continuous backcrossing with Y6 and self-crossing, a series of genetically-stable introgression lines were obtained. In this study,fourteen agronomic traits of 62 introgression lines were investigated. The correlation analysis,principal component analysis,regression analysis, and cluster analysis were used to explore the relationship between agronomic traits and the main factors affecting the yield per plant. The results revealed significant differences in agronomic traits among the introgression lines.The yield per plant was positively correlated with the silique number per plant,the number of effective branches, and seeds per silique,but negatively correlated with the height of the primary branch. Principal component analysis showed that,four principal components could reflect large proportion of the genetic variations that observed in 14 agronomic traits,which are mainly related to traits such as silique number,plant height,silique length,and 1000-grain weight. Regression analysis showed that 1000-grain weight,seeds per silique,secondary effective branch number,plant height,and silique number per plant had pivotal effects on yield per plant. Some introgression lines with excellent traits were characterized based on the agronomic characteristics. The silique number of L8 significantly increased. The branch number of L3 and L7 significantly increased. The 1000-grain weight of L42 and L49 significantly increased obviously. The yield per plant of L3 and L8 significantly increased. Near infrared spectroscopy was used to analyze the seed quality characters of the introgression lines,and the results revealed that glucosinolates,erucic acid,and linolenic acid showed great variation,which could be used to select the materials with advantageous quality characters. Taken together,this study provided a successful example of distant hybridization which served as an effective approach for the innovation of the B. napus germplasm resources and the improvement of B.napus yield. The results of this study laid a theoretical basis for B.napus breeding with these germplasm resources.
Key words:  Brassica napus L.  Sinapis alba L.  somatic hybrid  agronomic traits  yield per plant  qualitative characters

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