日本引进芸薹属白菜型种质资源耐镉性评价鉴定
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农业农村部油料作物生物学与遗传育种重点实验室 / 中国农业科学院油料作物研究所,武汉 430062

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国家自然科学基金(31470088);国家重点研发计划项目(2016YFD0100202)


Evaluation of Cadmium Tolerance in Brassica rapa L. Resources Imported from Japan
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Affiliation:

Key Laboratory of Biology and Genetic Improvement of Oil Crops/Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan 430062

Fund Project:

The National Natural Science Foundation of China (31470088),The National Key Program for Research and Development (2016YFD0100202)

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    摘要:

    为了丰富我国芸薹属资源中低镉资源的多样性,本研究对日本引进的 79 份白菜型资源在幼苗期进行较高(30 mg/L) 和中度(10 mg/L)的镉胁迫处理,从而对其开展耐镉性和镉积累特性研究,相较于传统采用镉污染的土壤进行培养的方法,具 有省时、省力、稳定性高等优点。结果显示,79 份资源的耐镉性变异丰富,较高(30 mg/L)和中度(10 mg/L)的镉胁迫处理下 筛选到稳定耐镉和镉敏感的材料各 3 份。镉积累特性分析发现,稳定耐镉材料地上部和胚根的 Cd 含量均显著低于镉敏感材 料,并且 D069 胚根和地上部的镉含量都显著低于其他材料,D125 的迁移系数相对较低,具有较高的育种利用价值。本研究 筛选得到的 D069 和 D125 两份耐镉和低积累资源丰富了国内芸薹属资源中低积累镉的基因库,将极大促进芸薹属污染安全 新品种培育进程,可有效推动镉污染农田的安全利用效率。

    Abstract:

    In order to enrich the diversity of low and medium cadmium resources in Brassica resources in China, 79 Brassica resources introduced from Japan were treated with high(30 mg/L)and medium(10 mg/L)cadmium stress at seedling stage,so as to carry out the research on cadmium tolerance and accumulation characteristics of Brassica resources. Compared with the traditional method of cultivating soil polluted by cadmium,it has the advantages of time-saving,labor-saving and stability High quality. The results showed that 79 resources had rich variation in cadmium tolerance. Three stable and three cadmium sensitive materials were selected under high(30 mg/L)and medium(10 mg/L)cadmium stress. The analysis of Cd accumulation characteristics showed that the CD content of stable CD tolerant material was significantly lower than that of CD sensitive material,and that of d069 was significantly lower than that of other materials,and the migration coefficient of D125 was relatively low,which had high breeding value. In this study,d069 and D125,two gene pools of cadmium tolerance and low accumulation resources,rich in domestic Brassica resources and low accumulation of cadmium,will greatly promote the breeding process of new varieties of Brassica pollution safety,and effectively promote the safe utilization efficiency of cadmium polluted farmland.

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引用本文

管文杰,张付贵,肖欣,等.日本引进芸薹属白菜型种质资源耐镉性评价鉴定[J].植物遗传资源学报,2020,21(1):105-112.

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  • 收稿日期:2019-11-08
  • 最后修改日期:2019-11-08
  • 录用日期:2019-11-15
  • 在线发布日期: 2020-01-17
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