甘蓝型油菜角果特异白化种质的遗传和生理特性
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安徽省农业科学院作物研究所 / 国家油料改良中心合肥油菜分中心,合肥 230031

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国家重点研发计划项目 (2016YFD0100202-16、2018YFD0100604);国家科技重大专项 (2018ZX08020001-005)


Genetic and Physiological Characteristics of Brassica napus Germplasm Resources Showing Albino Silique
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Affiliation:

Institute of Crop Science,Anhui Academy of Agricultural Science/National Oil Crops Improvement Center, Hefei Rapeseed Sub-center,Hefei 230001

Fund Project:

the National Key Research and Development Project (2016YFD0100202-16、2018YFD0100604);National Science and Technology Major Project (2018ZX08020001-005)

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    摘要:

    本研究对在甘蓝型油菜中发现的一种新型角果特异白化种质的形态特征、遗传及生理特性进行了研究,结果表明: 该种质具经济器官特异性白化的特点,即营养器官正常,生殖生长阶段所有的经济器官如花蕾、花、角果、种子均表现为白化突 变。白化株结实性差,纯合白化种子未发育成熟就干瘪萎缩。通过对该角果特异白化株与野生型油菜杂交创建的分离群体分 离比进行统计,表明该性状为 1 对核基因控制的不完全显性突变。纯合白化株系中几乎没有叶绿素和类胡萝卜素,但有少量 生育酚的合成,光合速率显著低于野生型或接近于零,超微结构观察也未发现有完整叶绿体结构。结合已有研究报道,初步推 断该特异白化种质为类胡萝卜素合成调控基因或决定叶绿体发育基因发生突变所引起。因角果是油菜最重要的光合器官,对 油菜产量形成起着至关重要的作用,本研究为进一步揭示叶绿体发育以及角果光合作用形成的分子生理奠定了基础。

    Abstract:

    Chlorophyll is the most important organism where plant photosynthesis takes place,and plays a curial role in plant growth and crop yield production. Albino plants showed complete loss of chlorophyll. In this study,the genetic,morphological and physiological characteristics of an albino silique mutant in Brassica napus were carried out. No chlorophyll was found on the reproductive tissues such as the buds,flowers,siliques and developing seeds,while vegetative tissues including leaves and stems kept green as wild type plants. This mutant that are heterozygous at albino locus exhibited lower fertility,while the mutant being homozygous were completely sterile. By genetic and phenotypic analysis of reciprocal F1 as well as was observed and F2 segregating population,the silique showing albino phenotype was controlled by one pair of nuclear gene in an incomplete dominant manner. Almost no chlorophyll and carotenoids,but a small amount of tocopherol were detected in homozygous albino mutant,and no intact chloroplast structure was found through Transmission Electron Microscope. The photosynthetic rate was also significantly lower than that of the wild type or close to zero. Gained from the knowledge in previous studies,the preliminary result suggested that the albino phonotype in this mutant was likely resulted from the mutations of the genes involved in the synthesis of carotenoids or the development of chloroplast. Thus,this work laid a foundation for future isolating the albino gene and further revealing the molecular mechanism of chloroplast development and silique photosynthesis.

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江莹芬,吴新杰,费维新,等.甘蓝型油菜角果特异白化种质的遗传和生理特性[J].植物遗传资源学报,2020,21(1):113-120.

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  • 收稿日期:2019-10-31
  • 最后修改日期:2019-11-06
  • 录用日期:2019-11-12
  • 在线发布日期: 2020-01-17
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