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紫薇属及近缘属13种植物基因组大小测定
刘婷婷1, 梁晓涵1, 张烨1, 程堂仁1, 王佳1, Mengmeng Gu2, 张启翔1, 潘会堂1
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(1花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/城乡生态环境北京实验室/北京林业大学园林学院,北京 100083;2Department of Horticultural Sciences, Texas A&M AgriLife Extension, College Station, TX 77843-0100, USA)
摘要:
为探究千屈菜科紫薇属(Lagerstroemia)、黄薇属(Heimia)及千屈菜属(Lythrum)之间的亲缘关系,本文利用 流式细胞术以二倍体白菜(Brassica rapa L.)和黄瓜(Cucumis sativus L.)为内标对紫薇属 8 种 2 品种、黄薇属 2 种和千屈菜 属 1 种进行了基因组大小测定,利用染色体压片技术获得 2 种黄薇属植物和千屈菜的染色体核型参数。结果表明,10 份紫薇 属植物的基因组大小在 341.00±2.00 Mbp 到 370.00±8.89 Mbp 之间;黄薇(Heimia myrtifolia Cham. et Schltdl.)和柳叶黄薇(H. salicifolia Link)基因组分别为 414.67±5.77 Mbp 和 420.00±7.00 Mbp,染色体数目均为 16,核型公式分别为 2n=16=6sm+8m+2st 和 2n=16=2sm+14m;千屈菜(Lythrum salicaria L.)的基因组为 1294.00±30.32 Mbp,染色体数目为 54,核型公式为 2n=54=31sm+15m+8st。本研究首次报道了千屈菜科 13 种植物的基因组大小,以及 2 种黄薇属植物和千屈菜的核型,研究结 果为千屈菜科植物基因组学和遗传进化研究奠定了基础。
关键词:  紫薇属  黄薇属  千屈菜属  基因组大小  染色体  流式细胞术
DOI:10.13430/j.cnki.jpgr.20191024004
投稿时间:2019-10-24修订日期:2019-12-30
基金项目:北京市科技计划课题(Z181100002418006);国家自然科学基金项目(31470695)
Genome Size Determination of 13 Taxa of Lagerstroemia and Two Closely Related Genera
LIU Ting-ting1, LIANG Xiao-han1, ZHANG Ye1, CHENG Tang-ren1, WANG Jia1, Mengmeng Gu2, ZHANG Qi-xiang1, PAN Hui-tang1
(1Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/College of Landscape Architecture, Beijing Forestry University, Beijing, 100083; 2 Department of Horticultural Sciences, Texas A &M AgriLife Extension, College Station, TX 77843-0100, USA)
Abstract:
In order to explore the genetic relationship among Lagerstroemia, Heimia and Lythrum of the family Lythraceae, the genome sizes of eight species and two cultivars of Lagstroemia, of two species of Heimia and of Lythrum salicaria L. were estimated by flow cytometry method with Brassica rapa L. and Cucumis sativus L. as inner calibration. The chromosome number and karyotype parameters of the two Heimia species and of Lythrum salicaria were obtained using traditional chromosome tableting. The results showed that the average genome size of Lagerstroemia species and cultivars ranged from 341.00±2.00 Mbp to 370.00±8.89 Mbp. The average genome size of Heimia myrtifolia Cham. et Schltdl. and H. salicifolia Link were 414.67±5.77 Mbp and 420.00±7.00 Mbp respectively, and the karyotype formulas of the two species were 2n=16=6sm+8m+2st and 2n=16=2sm+14m. The average genome size of Lythrum. salicaria was 1294.00±30.32 Mbp, and the karyotype formula was 2n=54=31sm+15m+8st. In this study, the genome size of 13 taxa in the Lythraceae, and the karyotypes of two Heimia species and of L. salicaria were reported for the first time. The results of our research laid a foundation for research on genomics and genetics of the Lythraceae.
Key words:  Lagerstroemia  Heimia  Lythrum  genome size  chromosome  flow cytometry

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