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小麦-中间偃麦草6JS/6B代换系的分子细胞学鉴定
罗小军1, 乔麟轶2, 李欣2, 李光蓉3, 郭慧娟2, 阎晓涛2, 张树伟2, 常利芳2, 闫金龙4, 畅志坚2, 张晓军2
0
(1.山西大学生物工程学院;2.山西省农业科学院作物科学研究所/作物遗传与分子改良山西省重点实验室/农业部黄土高原作物基因资源 与种质创制重点实验室;3.电子科技大学生命科学与技术学院;4.山西省农业科学院谷子研究所)
摘要:
将近缘植物的抗病基因导入小麦是改良小麦抗病性的重要途径之一,对其外源染色体进行准确鉴定能够提高外源 基因的选择与利用效率。本研究分别利用小麦白粉、条锈菌生理小种接种、荧光原位杂交和分子标记的方法对来源于中间偃 麦草的部分双二倍体 TAI7047 为中间亲本创制的新种质 CH357 进行了鉴定分析。结果显示,CH357 是一个小麦-中间偃麦草 6JS/6B 代换系,兼抗小麦白粉、条锈 2 种病害,其抗性可能来源于中间偃麦草的 6JS 染色体,可以作为一个小麦白粉病和条锈 病新抗源进行小麦抗性遗传改良。基于中间偃麦草第 6 同源群 Contig 序列开发了 160 个 STS 标记,其中 8 个可作为识别小麦- 中间偃麦草异代换系 CH357 中 6JS 染色体/片段的特异标记,为中间偃麦草 6JS 染色体/片段的鉴定提供较为经济和方便的检测 手段。
关键词:  中间偃麦草  代换系  染色体  STS  分子标记
DOI:10.13430/j.cnki.jpgr.20191010003
投稿时间:2019-10-10修订日期:2019-11-15
基金项目:国家重点研发计划(2016YFD0102004);山西省重点研发计划(201803D221018-5, 201703D211007, 201803D421020);山西省农业科学院项目 (YGG17123, YCX2018D2YS01);山西省重点科技创新平台(201605D151002)
Molecular and Cytogenetic Identification of Wheat-Th. intermedium 6JS/6B Substitution Line
LUO Xiao-jun1, QIAO Lin-yi2, LI Xin2, LI Guang-rong3, GUO Hui-juan2, YAN Xiao-tao2, ZHANG Shu-wei2, CHANG Li-fang2, YAN Jin-long4, CHANG Zhi-jian2, ZHANG Xiao-jun2
(1.College of Bio-engineering,Shanxi University;2.Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture;3.College of Life Science and Technology, University of Electronic Science and Technology of China;4.Millet Research Institute, Shanxi Academy of Agricultural Sciences)
Abstract:
Transforming resistance genes of wild relatives into elite cultivars is an effective approach to improve the wheat disease resistance. Identification of the alien chromosomes by deployment of a rapid and precise method becomes important in order to simplify the process on selection and utilization of the favorable genes. In this study, CH357, a new wheat germplasm resource which was derived from wheat-Thinopyrum intermedium partial amphiploid TAI7047, was subjected for tests upon infections of Powdery mildew and Stripe rust, as well as the evaluation by fluorescent in situ hybridization (FISH) and molecular markers detection. The results showed that CH357 contained a wheat-Th. intermedium 6JS/6B chromosome substitution. This genotype was tested to be resistant against wheat powdery mildew and stripe rust, and the resistances were likely caused by 6JS chromosome of Th. Intermedium, thus raising a potential for wheat resistance breeding. Furthermore, 160 STS markers were developed based on the Contigs sequences of Th. intermedium group 6 genome. Out of that, eight markers were found to specifically amplify the alien chromosome of CH357. The PCR-based assay will provide a user-friendly method for identifying the 6JS chromosome or fragments of Th. intermedium in wheat.
Key words:  Th.intermedium  substitution line  chromosome  STS  molecular marker

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