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400份小麦品种(系)条锈病成株期抗性鉴定与评价
尉法刚, 王光浩, 王长有, 张宏, 刘新伦, 田增荣, 朱建峰, 陈春环, 吉万全, 王亚娟
0
(西北农林科技大学农学院/农业部作物基因资源与种质创制陕西科学观测实验站)
摘要:
发掘新抗源,不断拓宽抗病遗传资源,是确保农业生产可持续发展的重要战略储备。为明确400个小麦品种(系) 的抗条锈表现及抗条锈基因分布状况,本研究利用关中地区优势混合小种(CYR31、CYR32和Su11-14)在田间进行成株期条 锈病抗性鉴定,同时以Yr5、Yr9、Yr10、Yr15、Yr17、Yr18和Yr26已知抗条锈病基因的分子标记进行检测筛查,综合分析供试 材料可能携带的抗病基因。结果表明,在400份材料中,成株期对混合菌种表现高抗至免疫(IT=0~1)的品种(系)有177份, 占44.25%;中抗(IT=2)品种(系)62份,占15.5%;中感或高感(IT=3/4)品种(系)161份,占40.25%。结合抗病表现和已 知Yr基因分子检测分析表明:供试小麦中121份材料携带Yr5,占30.25%;96份携带Yr9,占24%;10份携带Yr10,占2.5%;19 份携带Yr15,占4.75%;150份携带Yr17,占37.5%;15份携带Yr18,占3.75%;129份携带Yr26,占32.25%。其中定西24、N7187 等25份材料未发现携带Yr5、Yr9、Yr10、Yr15、Yr17、Yr18和Yr26,推测可能含有其它未知抗性基因或新基因。该研究结果建 立了小麦条锈病抗源鉴定和评价体系,筛选出177份具有不同抗病性特征的抗源材料,其中25份可能含有新抗源,为进一步培 育抗条锈病新品种奠定了基础。
关键词:  小麦  条锈病  成株期  抗病基因  分子检测
DOI:10.13430/j.cnki.jpgr.20191009001
投稿时间:2019-10-09修订日期:2020-01-14
基金项目:国家重点研发计划(2016YFD0100102);作物种质资源保护项目(2019NWB036-02-1)
Evaluation and Identification of Adult Resistance to Stripe Rust From 400 Wheat Varieties (Lines)
YU Fa-gang, WANG Guang-hao, WANG Chang-you, ZHANG Hong, LIU Xin-lun, TIAN Zeng-rong, ZHU Jian-feng, CHEN Chun-huan, JI Wan-quan, WANG Ya-juan
(College of Agronomy/Ministry of Agriculture Crop Genetic Resources and Seed Creation Shaanxi Scientific Observation And Experimental Station, Northwest Agricultrue and Forestry University, Yang ling 712100, Shaanxi)
Abstract:
Exploring new sources of resistance and widening genetic resources of disease resistance are important strategic reserves to ensure the sustainable development of agricultural production. In order to get it clear about the resistance to stripe rust and the distribution of stripe rust resistance genes in 400 wheat varieties (lines), the resistance to stripe rust was identified on adult plants in the field by using the dominant mixed races (CYR31,CYR32 and Su11-14) in Guanzhong area, and Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 which are known as stripe rust resistance genes were detected and screened by molecular markers in this study for comprehensive analysis of resistance genes that may be carried by tested materials. The results showed that out of the 400 materials, 177 (44.25%) were highly resistance to immunity (IT=0~1) to mixed races at adult stage, 62 (15.5%) were moderate resistance (IT=2) and 162 (40.25%) were medium or high susceptible (IT=3/4). Combined with disease resistance performance and molecular analysis of known Yr gene, the results showed that Yr5 was carried by 121 (32.5%) wheat materials, Yr9 was carried by 96 (24%) materials,Yr10 was carried by 10 (2.5%) materials, Yr15 was carried by 19 (4.75%) materials, Yr17 was carried by 150 (37.5%) materials, Yr18 was detected in 15(3.75%) materials, and Yr26 was detected in 129 (32.5%) materials, respectively. But Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 were not found in 25 materials such as Dingxi 24 and N7187 among the 400, they might contain other unknown resistance genes or new genes. The results of the study established the identification and evaluation system of resistance to wheat stripe rust, and 177 resistant materials with different resistance characteristics were selected out, of which 25 might contain new resistance sources, which laid a foundation for further cultivation of new varieties resistant to stripe rust
Key words:  wheat  stripe rust  adult plant  resistance gene  molecular detection

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