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山东省3个野生大豆居群生育期基因遗传多样性分析
刘月1, 齐广勋2, 桑永生1, 王英男3, 李玉秋3, 王玉民3, 刘晓冬3, 赵洪锟2, 袁翠平3, 董英山3
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(1.吉林农业大学农学院;2.吉林省农业科学院作物资源研究所;3.吉林省农业科学院大豆研究所)
摘要:
以来自山东省 3 个居群的 137 份野生大豆种质资源为试验材料,利用等位基因特异性 DNA 标记进行生育期 E1-E4 基因型鉴定及遗传多样性分析。结果表明,山东省野生大豆生育期基因 E1-E4 基因中,E1、E4 位点上,只检测出 1 个等位 变异,皆为显性基因型 E1、E4;E2、E3 位点上变异较为丰富,分别以 E2-in、E3-Ha 为主;共发现 5 种基因型组合,其中 E1E2-dlE3-HaE4 基因型为优势组合。三个居群遗传变异不尽相同,临沂居群 E2、E3 位点以 E2-in、E3-Mi 为主,共有 4 种 基因型组合,基因型组合以 E1E2-inE3-MiE4 为主。蓬莱和荣成居群,E2、E3 位点以 E2-dl、E3-Ha 为主,共有 3 种基因型 组合,以 E1E2-dlE3-HaE4 为主。在遗传多样性方面,山东荣成和蓬莱的亲缘关系较近,与临沂亲缘关系较远。遗传多样性 最高的为临沂居群,其次是蓬莱居群,荣成居群。本研究结果可为山东省野生大豆种质资源利用提供理论依据,具有重要的 应用价值。
关键词:  野生大豆  E基因  遗传多样性  相关性
DOI:10.13430/j.cnki.jpgr.20190909001
投稿时间:2019-09-09修订日期:2019-11-12
基金项目:吉林省农业科技创新工程(CXGC2018JC003);吉林省农业科技创新工程(CXGC2017ZY006);吉林省农业科技创新工程人才基金项目(C92070402)
Genetic Diversity Analysis of Wild Soybean Resources from Three Populations of Shandong Province by Using the Functional Markers Targeting the Growth Period-Related Genes
LIU Yue1, QI Guang-xun2, SANG Yong-sheng1, WANG Ying-nan3, LI Yu-qiu3, WANG Yu-min3, LIU Xiao-dong3, ZHAO Hong-kun2, YUAN Cui-ping3, DONG Ying-shan3
(1.College of Agriculture, Jilin Agricultural University;2.Crop Germplasm Resources Institute, Jilin Academy of Agricultural Sciences,Jilin Gongzhuling;3.Soybean Research Institute, Jilin Academy of Agricultural Sciences)
Abstract:
The genetic diversity analysis of 137 wild soybean accessions from three populations of Shandong province were analyzed by allele-special DNA markers. One dominant allele was detected at E1 and E4 loci, respectively, while the haplotypes E2-in and E3-Ha were found to be predominant at E2 and E3 loci, respectively. Out of five genotypic combinations, the genotype carrying E1E2-dlE3-HaE4 was predominant. By further analyzing the diversity among the three populations, four genotypic combinations were detected in Linyi population. The genotypes carrying E1E2-inE3-MiE4 were often detected, while E2-in and E3-Mi were major alleles at E2 and E3 loci, respectively. Three genotypic combinations were observed in both Penglai and Rongcheng populations, while E1E2-dlE3-HaE4 was often detected. The alleles E2-dl and E3-Ha were superiority allele on E2 and E3 loci, respectively. A similarity on genetic diversity was found between Rongcheng and Penglai populations, while both were differing with Linyi population that represented a highest genetic diversity. Taken together, the results gained from this study might provide insights for the utilization of wild soybean germplasm resources in Shandong Province.
Key words:  wild soybean  E gene  genetic diversity  correlation

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