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河北省小麦品种和种质资源抗白粉病鉴定 与抗病基因分子标记检测
延荣1, 耿妙苗1, 李晓静2, 安浩军2, 温树敏1, 刘桂茹1, 王睿辉1
0
(1.河北农业大学 / 华北作物种质资源研究与利用教育部重点实验室;2.保定市农业科学院)
摘要:
白粉病是河北省小麦生产的重要常发病害,明确小麦审定品种和高代品系中所携带的抗病基因对合理利用和布局 已知抗源、实现对小麦白粉病的有效防控具有重要意义。本研究结合人工接种白粉病菌株 E09 和 E20 与抗病基因连锁(或共 分离)标记对 1956-2018 年间河北省 371 份小麦材料(含审定品种 256 份,高代品系 115 份)进行苗期抗白粉病鉴定和抗病 基因检测。结果表明:供试材料中,抗 E09 的材料占 6.2%,抗 E20 的占 11.9%,兼抗两个菌株的材料占 4.9%;部分材料携 带 Pm1c、Pm2、Pm4b、Pm21、Pm24 和 Pm35 基因,未检测到 Pm12 基因。Pm8 基因在供试材料中所占比例较高,接近 50.0%。 供试材料中抗病审定品种比例远大于高代品系,说明小麦抗白粉病种质创新仍为当务之急,需要引起重视。在用连锁或共分 离标记进行抗病基因检测时,通过计算某基因对两个菌株抗病反应型与标记检测结果一致的材料比例,发现 Pm12、Pm21 和 Pm35 等基因的标记检测效率较高,同时这些基因的标记也方便使用,可优先考虑用这些标记检测目的基因。
关键词:  河北小麦  审定品种  品系  白粉病  抗性基因  分子标记
DOI:10.13430/j.cnki.jpgr.20190902001
投稿时间:2019-09-02修订日期:2019-11-24
基金项目:国家自然科学基金(30600389、31371617)
Phenotyping and Marker-assisted Gene Identification of Powdery Mildew Resistance in Wheat Commercial Varieties and Germplasm Resources Collected from Hebei Province
YAN Rong1, GENG Miao-miao1, LI Xiao-jing2, AN Hao-jun2, WEN Shu-min1, LIU Gui-ru1, WANG Rui-hui1
(1.Hebei Agricultural University /Key laboratory of Research and Utilization of Crop Germplasm Resources in North China, Ministry of Education;2.Baoding Academy of Agricultural Sciences)
Abstract:
Powdery mildew, which is caused by biotrophic fungus Blumeria graminis f. sp. tritici (Bgt), is an important epidemic disease threating the wheat production in Hebei province of China. Identification of disease-resistance genes in the commercially authorized varieties and breeding lines is of great significance for efficient utilization and regional distribution of known resistance sources and effective managing of wheat powdery mildew. In this study, 371 wheat accessions (including 256 commercial varieties from 1956 to 2018 and 115 breeding lines) from Hebei province were tested for resistance to powdery mildew at seedling stage with independent inoculation with two isolates of E09 and E20, as well as marker-assisted classification of eight genetically-mapped Pm genes. The results showed that 6.2% and 11.9% of accessions were tested to be resistant to E09 and E20, respectively, while only 4.9% were resistant to both isolates. Marker-assisted selection showed that resistance genes Pm1c, Pm2, Pm4b, Pm21, Pm24 and Pm35 were found in some accessions, while Pm12 was not detected. Almost half of the accessions were found to carry Pm8. It was shown that resistance carrying accessions of resistant authorized varieties were found to be much higher than that of breeding lines, indicating that genetic germplasm improvement enhancement for powdery mildew resistance is an urgent task at present and should be paid more attention. When using linkage or co-segregation markers for Pm gene detection, by means of the method that the number of materials that the resistant reaction type consistent with result of the marker detection occupies the total number of materials detected by the linkage marker of the gene, finding markers linked to Pm12, Pm21 and Pm35 is highly effective. Taking advantage of the user-friendly markers targeting these resistance genes, marker-assisted selection for resistance-carrying lines can be conducted in advance in contrast to a test for resistance.
Key words:  Hebei wheat  commercial varieties  breeding lines  Blumeria graminis f. sp. tritici  resistance genes  molecular marker

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