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基于SSR标记的云南省核桃种质资源遗传多样性研究和核心种质构建
吴涛1, 陈少瑜1, 肖良俊2, 宁德鲁2, 潘莉2, 贺娜2, 朱云凤2
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(1.云南省林业科学院 国家林业局云南珍稀濒特森林植物保护和繁育重点实验室/云南省森林植物培育与开发利用重点实验室;2.云南省林业科学院 经济林研究所)
摘要:
利用 SSR 分子标记技术对云南省 14 州市 1061 份核桃种质资源进行遗传多样性分析,在此基础上,通过最小距离 逐步取样法构建核心种质并进行了核心种质的多样性检测。结果表明,19 对 SSR 引物共检测到 322 个等位基因,每对引物平 均 16.95 个,基因多样性指数(I)平均为 1.3375,期望杂合度(He)平均为 0.6040,Nei’s 遗传多样性指数 (Nei)平均为 0.6043,数据表明云南核桃种质资源具有较为丰富的遗传多样性。遗传多样性在各州市分布不均,其中迪庆州核桃资源的遗 传多样性相对最高,相对最低的是临沧地区。基于遗传距离的 UPGMA 聚类结果,以 30%~5%的取样比例分别构建初级种质(PC)、 次级种质(SC)、核心种质-1(CC-1)、核心种质-2(CC-2)等 4 个核心种质,经遗传多样性分析及 t 检验,最终确定 10.84% 取样比例、115 份样本量的核心种质-1(CC-1)为云南核桃资源的核心种质。
关键词:  核桃  SSR分子标记  种质资源  遗传多样性  核心种质
DOI:10.13430/j.cnki.jpgr.20190813001
投稿时间:2019-08-13修订日期:2019-09-10
基金项目:国家自然科学基金项目(31660214);云南省重大科技专项计划子专题(2018ZG002-01)
SSR Markers Based Genetic Diversity Analysis and Core Collection Construction of Walnut Germplasm in Yunnan Province
WU Tao1, CHEN Shao-yu1, XIAO Liang-jun2, NING De-lu2, PAN Li2, HE Na2, ZHU Yun-feng2
(1.Laboratory for Conservation and Breeding for Rare, Endangered and Endemic Forest Plants of State Forestry Administration/Yunnan Provincial Key Laboratory for Cultivation and Utilization of Forest Plants, Yunnan Academy of Forestry, Kunming;2.Institute of Economic Forest Trees, Yunnan Academy of Forestry, Kunming)
Abstract:
Genetic diversity of 1061 walnut germplasm from 14 prefectures and cities of Yunnan Province was analyzed by SSR molecular marker technique. On the basis of this, a core collection was constructed through least distance stepwise sampling (LDSS) and tested for its diversity. The results showed that 322 alleles were detected by 19 pairs of SSR primers (on average 16.95 per pair of SSR primer). The means of Shannon’s information index (I), expected heterozygosity (He) and Nei’s gene diversity (Nei) were respectively 1.3375, 0.6040 and 0.6043, which indicated a relatively high genetic diversity of the germplasm. The genetic diversity distributed differently among the 14 prefectures and cities. Diqing Prefecture had the highest genetic diversity while Lincang had the lowest. Sampling rates of 30%-5% were taken for construction of the primary core collection, secondary core collection, core collection-1 and core collection-2, on the basis of UPGMA clustering results. With the genetic diversity analysis and t-test, the core collection-1 with 10.84% sampling rate and 115 samples was determined to be the core collection of walnut germplasm in Yunnan.
Key words:  Juglans sigillata  SSR molecular markers  germplasm resources  genetic diversity  core collection

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