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玉米穗部性状及其一般配合力的关联分析
刘文童, 监立强, 郭晋杰, 赵永锋, 黄亚群, 陈景堂, 祝丽英
0
(河北农业大学农学院)
摘要:
穗部性状是影响玉米产量的重要性状,一般配合力是评价玉米自交系利用价值的重要指标。为解析穗部性状及其 一般配合力的遗传基础,本研究对 248 份玉米自交系组成的自然群体和以其中 100 份自交系为母本按照 NCⅡ遗传交配设计与 4 个测验种(Mo17、昌 7-2、E28和郑 58)组配的 400 份 F1 杂交组合的穗部性状进行研究,并利用分布于全基因组的 83057 个 SNP 标记进行穗部性状及其一般配合力的关联分析。结果表明,穗长、穗粗 2 个穗部性状基因型间、环境间差异达 极显著水平,其广义遗传率分别为 81.22%和 87.70%。母本间、父本间及不同杂交组合间穗长、穗粗差异均达极显著水平, 在基因型方差中特殊配合力方差所占比例较大。利用 2 年 2 点 4 个环境下的数据分别进行关联分析,检测到 34 个性状-SNP 关联位点,利用 BLUP 值检测到 7 个性状-SNP 关联位点。这些关联位点可解释的表型变异在 0.01%~19.42%,其中有 4 个位 点的表型贡献率大于 10%,未检测到穗部性状本身与一般配合力性状的相同关联位点。基于该群体的 LD 衰减距离在显著关 联位点上下游各 120 kb 范围内进行候选基因搜素,共发现 158 个候选基因,推测可能候选基因涉及泛素代谢相关基因 (GRMZM2G360374、GRMZM2G049568、GRMZM2G178120),半乳糖苷酶(GRMZM2G178106),丝氨酸苏氨酸蛋白激酶 (GRMZM2G127050),赖氨酸和组氨酸特异性转运体(GRMZM2G116004)。研究结果为解析玉米穗长和穗粗及其一般配 合力的遗传基础和分子辅助选择育种提供了参考。
关键词:  玉米  穗部性状  一般配合力  关联分析  候选基因
DOI:10.13430/j.cnki.jpgr.20190801001
投稿时间:2019-08-01修订日期:2019-08-22
基金项目:国家重点研发计划(2018YFD0300501)
Association Mapping of Ear-Related Traits and Their General Combining Ability in Maize
LIU Wen-tong, JIAN Li-qiang, GUO Jin-jie, ZHAO Yong-feng, HUANG Ya-qun, CHEN Jing-tang, ZHU Li-ying
(College of Agronomy, Hebei Agricultural University)
Abstract:
Ear-related traits have an important impact on maize yield and general combining ability (GCA) is an important indicator for assessing maize inbred lines. 248 inbred lines of maize and 400 F1 hybrids derived from combinations with 100 lines selected randomly from the 248 inbred lines as the female parents and four elite inbred lines (‘Mo17’, ‘Chang 7-2’, ‘E28’ and ‘Zheng 58’) as the testers according to the NCII genetic mating design were used to study the ear-related traits. The genome-wide association mapping of ear-related traits and their GCA was performed using 83057 SNP markers. The results indicated that the ear-related traits were significantly different among the genotypes and the environments. The broad-sense heritability of ear length (EL) and ear diameter (ED) were 81.22% and 87.70%, respectively. Combining ability analysis indicated that there were significant differences among females, males and combinations for the two traits. The contribution rate of special combining ability was greater than general combining ability. 34 significant SNP-trait associations were identified using the data of 4 environments (2 locations in 2 years) respectively, and 7 significant SNP-trait associations were detected using the data of best linear unbiased prediction. Each locus can explain phenotypic variation ranged from 0.01% to 19.42% and 4 of them had PVE value greater than 10%. The same SNPs of the ear-related traits and GCA were not detected simultaneously. Based on the LD decay distance, candidate genes were identified in the range of the 120 kb upstream and downstream of significantly associated loci, and 158 genes were obtained. The putative candidate genes were involved in ubiquitin metabolism (GRMZM2G360374, GRMZM2G049568, GRMZM2G178120), galactosidase (GRMZM2G178106), serine threonine protein kinase (GRMZM2G127050), lysine and histidine specific transporters (GRMZM2G116004). The study provides a reference for the analysis of the genetic basis and molecular-assisted selection breeding of maize ear-related traits and general combining ability.
Key words:  maize  ear-related traits  general combining ability  association mapping  candidate gene

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