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基于染色体置换系的普通野生稻耐盐性QTL定位
许睿1, 张莉珍1, 谢先芝2, 王艳艳1, 黄婧芬1, 郑晓明1, 张丽芳1, 程云连1, 郑崇珂2, 乔卫华1, 杨庆文1
0
(1.中国农业科学院作物科学研究所;2.山东省水稻研究所)
摘要:
本研究分别利用 SSR 标记、InDel 标记以及简化基因组测序技术对一套以普通野生稻为供体亲本,9311 为受体亲 本的染色体置换系进行基因型鉴定,并通过对其不同生育期的耐盐性鉴定,共发掘 2 个与发芽期耐盐相关的 QTL,13 个与苗 期耐盐性相关的 QTL。其中与苗期存活率相关 QTL qSSR5.1、苗期耐盐等级相关 QTL qSSG5.1 均被定位于同一位点,该位点 对苗期存活率、苗期耐盐等级均具有增效作用,贡献率分别为6.36%、8.13%。在此QTL内包含与非生物胁迫相关基因OsDi19-1。 经序列比对发现,OsDi19-1 启动子区域在两亲本间存在较大差异,且受到盐胁迫时该基因表达量上升。同时,鉴定出水稻芽 期耐盐的优异种质资源 CSSL72、苗期耐盐的优异种质资源 CSSL23、CSSL153,为水稻育种中耐盐性的改良提供了新的种质资源。
关键词:  普通野生稻  耐盐  染色体片段置换系
DOI:10.13430/j.cnki.jpgr.20190627004
投稿时间:2019-06-27修订日期:2019-10-12
基金项目:国家自然科学基金(31471471)
QTL Mapping Revealed Salt Tolerance Traits from O. rufipogon using Chromosome Segment Substitution Lines
XU Rui1, ZHANG Li-zhen1, XIE Xian-zhi2, WANG Yan-yan1, HUANG Jing-fen1, ZHENG Xiao-ming1, ZHANG Li-fang1, CHEGN Yun-lian1, ZHENG Chong-ke2, QIAO Wei-hua1, YANG Qing-wen1
(1.Institute of CropSciences,Chinese Academy of Agricultural Sciences;2.Shandongriceresearch institute)
Abstract:
Within this study, a QTL mapping of salt tolerance was conducted by using a population of chromosome segment substitution lines (CSSLs), which were generated from common wild rice as donor parent and cultivated rice (9311) as receive parent. Genotyping for CSSLs were performed with types of markers (SSR, InDel and genotyping-by-sequencing markers). The phenotypic datasets were assessed by systemically evaluating the salt tolerance at different growth periods. As a result, two QTLs at germination stage and 13 QTLs at seedling stage were obtained. Among QTLs, qSSR5.1 and qSSG5.1 were found at the same locus, which synergistically contributed to the seedling survival rate (6.36%) and salt tolerance grade (8.13%). Within the target interval, one candidate gene OsDi19-1 which associated with abiotic stress was found. Sequence alignment showed an obvious sequence variation between 9311 and wild rice in the promoter region of OsDi19-1. Notably, the line CSSL72 showed a higher germination rate, while CSSL23 and CSSL153 exhibited higher seedling rates. A down-regulation on gene expression was observed in CSSL23 and CSSL153, if relative to 9311 under salt treatment. Taken together, our data indicated that OsDi19-1 from wild rice might contribute to the salt tolerance, and these elite lines might become useful in future rice salt tolerance breeding.
Key words:  wild rice  salt-tolerance  CSSL, QTL

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