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基于SSR标记分析大豆疫霉根腐病抗源的遗传多样性
王帅1, 张子戌1, 齐广勋1, 王英男1, 赵洪锟1, 刘晓冬1, 袁翠平1, 王玉民1, 朴世领2
0
(1.吉林省农业科学院;2.延边大学农学院)
摘要:
丰富的遗传多样性可为大豆育种提供宽阔的遗传基础,本研究基于 35 对 SSR 标记,对 60 份东北地区大豆疫霉根腐病抗性品种进行了遗传多样性分析,共检测到 189 个等位基因,平均每个位点等位变异数 5.4 个,多态性信息含量指数(PIC)为 0.1550-0.8195,平均为 0.6636。遗传相似系数的变异范围为 0.31-0.74。利用 5 对高多态性 SSR 引物构建了 60 份抗性材料每份品种(系)的指纹图谱,这 5 对 SSR 引物构建的指纹图谱可以将 60 份大豆疫霉根腐病抗性材料逐一区分开。采用NTSYS2.10 基于遗传距离的聚类分析,将 60 份抗性材料分为 7 个类群,其中有 78.33%的抗性品种(系)的相似系数在 0.45-0.74间,表明遗传差异相对较窄,品种间遗传多样性水平较低。聚类分析与群体遗传结构分析结果有部分重合,均反应出不同地区的抗性材料间存在一定的渗透和交流。
关键词:  大豆  遗传多样性  大豆疫霉根腐病  SSR
DOI:10.13430/j.cnki.jpgr.20190611004
投稿时间:2019-06-11修订日期:2019-12-11
基金项目:国家重点研发计划(2016YFD0100201);吉林省农业科技创新工程人才基金项目(C92070403);吉林省农业科技创新工程重大项目 (CXGC2017ZD014)
Genetic Diversity of Resistance to Phytophthora Root Rot in Northeast China Based on SSR Markers
WANG Shuai1, ZHANG Zi-xu1, QI Guang-xun1, WANG Ying-nan1, ZHAO Hong-kun1, LIU Xiao-dong1, YUAN Cui-ping1, WANG Yu-min1, PIAO Shi-ling2
(1.Jilin Academy of Agricultural Sciences;2.College of Agronomy, Yanbian University)
Abstract:
The abundant genetic diversity provides a broad genetic basis for soybean breeding. Based on 35 pairs of SSR markers, the genetic diversity of 60 soybeans being resistant to Phytophthora Root Rot in Northeast China was analyzed. A total of 189 alleles were detected, with an average of 5.4 alleles per locus. The polymorphism information content index (PIC) was variable from 0.1550 to 0.8195 with an average of 0.6636. The genetic similarity coefficient varies from 0.31 to 0.74. The fingerprint map in each of resistant varieties was constructed using 5 pairs of highly polymorphic SSR primers. Using NTSYS2.10 clustering analysis based on genetic distance, sixty resistant genotypes were divided into 7 groups, of which 78.33% of resistant varieties (lines) had a similarity coefficient between 0.45 and 0.74. That indicated a relatively narrow genetic difference among these resistance-conferring genotypes. The results of cluster analysis and population genetic structure analysis partially overlap, suggesting the existence of multiple penetration and communication events between resistant materials in different regions.
Key words:  soybean  genetic diversity  Phytophthora sojae root rot  SSR

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