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玉米黄绿叶突变体表型鉴定及基因初步定位
刘忠祥, 寇思荣, 连晓荣, 杨彦忠, 王晓娟, 何海军, 周玉乾, 周文期
0
(甘肃省农业科学院作物研究所)
摘要:
叶色突变体是研究光合作用及叶绿体发育的重要材料。本研究以黄绿叶突变体ygl-F17138为材料,与玉米自交系B73进行杂交构建F2分离群体,进行遗传效应分析和基因初步定位。遗传分析表明,该突变性状由单个隐性核基因控制,且能稳定遗传。利用BSR-seq结合连锁分析的方法将该基因初步定位在第3条染色体上一个约9.2 Mb的区间内(chr3: 173087201-182203992),查询该区间内已知基因功能注释,未发现类似前人报道的调控黄绿叶性状基因,说明YGL-F17138基因是一个控制玉米黄绿叶发育候选基因。
关键词:  玉米  黄绿叶突变体  ygl-F17138  BSR-seq  遗传分析  基因定位
DOI:10.13430/j.cnki.jpgr.20190611002
投稿时间:2019-06-11修订日期:2020-01-06
基金项目:国家自然科学基金项目(31760390,31860384);国家重点研发计划(2018YFD0100202-3,2016YFD0100103-19)
Phenotypic Identification and Low-resolution Mapping of a Yellow-Green Leaf Mutant in Maize
LIU Zhong-xiang, KOU Si-rong, LIAN Xiao-rong, YANG Yan-zhong, WANG Xiao-juan, HE Hai-jun, ZHOU Yu-qian, ZHOU Wen-qi
(Crops Research Institute, Gansu Academy of Agricultural Sciences)
Abstract:
Leaf-color mutants are important materials for deciphering the photosynthesis and chloroplast development. In this study, a yellow-green leaf mutant was identified and designated ygl-F17138. By making a cross with the maize inbred line B73, we constructed a F2 segregation population for the genetic analysis and the preliminary gene mapping. Genetic analysis indicated that this yellow-green phenotype was genetically controlled by a recessive nuclear gene. By deployment of BSR-seq and the linkage analysis method, this gene was allocated to an interval of about 9.2 Mb on chromosome 3 (chr3: 173087201-182203992). According to the functional annotation of known genes in this interval, no genes which regulating yellow green leaf traits similar to those previously reported were found, indicating that YGL-F17138 gene is a candidate gene controlling yellow green leaf in maize.
Key words:  maize  yellow-green leaf mutant  ygl-F17138  BSR-seq  genetic analysis  gene mapping

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