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15个小麦农家品种抗叶锈基因分析
徐新玉, 段振盈, 张晓玲, 姚占军
0
(河北农业大学农学院/华北作物种质资源研究与利用教育部重点实验室)
摘要:
本研究旨在明确小麦农家品种中可能含有的抗叶锈病基因,为抗源的选择和利用提供理论依据。以 15 个小麦农家 品种、感病对照品种郑州 5389 和 36 个含有已知抗叶锈病基因的载体品种为材料,苗期接种 19 个具有鉴别力的叶锈菌生理小 种进行基因推导,同时利用 12 个与抗叶锈病基因紧密连锁的分子标记进行分析。为明确其成株期抗性,分别于 2016-2017 年 和 2017-2018 年在河北保定对小麦农家品种、感病对照品种郑州 5389 与慢锈品种 SAAR 进行田间接种,调查并记录田间严重 度及普遍率。基因推导和分子标记检测结果显示,在 15 个小麦农家品种中共检测到 7 个抗叶锈病基因,其中部分品种还有多 个抗性基因,比如红狗豆含有 Lr1 和 Lr46;黄花麦含有 Lr13 和 Lr34;大白麦含有 Lr14b 和 Lr26;洋麦含有 Lr37 和 Lr46;成 都光头含有 Lr34 和 Lr46;墨脱麦和西山扁穗含有 Lr26 和 Lr46。部分品种含有 1 个成株期慢叶锈病抗性基因,比如同家坝小 麦、武都白茧儿、边巴春麦-6、白花麦含有 Lr34;红抢麦、白扁穗和白火麦含有 Lr46。这些携带有效抗叶锈病基因的农家品 种,可为小麦抗叶锈病育种提供抗源
关键词:  小麦农家品种  叶锈病  基因推导  分子检测  慢锈品种
DOI:10.13430/j.cnki.jpgr.20190527003
投稿时间:2019-05-27修订日期:2019-07-10
基金项目:国家重点研发计划 (2017YFD300901)
Analysis of Leaf Rust Resistance Genes in 15 Wheat Landrace Cultivars
XU Xin-yu, DUAN Zhen-ying, ZHANG Xiao-ling, YAO Zhan-jun
(College of Agronomy, Agricultural University of Hebei / North China Key Laboratory for Germplasm Resources of China Education Ministry)
Abstract:
The objective of this study is to determine the potential resistance genes in wheat landraces and provide reference information for the selection and utilization of leaf rust resistance resources. In this study, 15 wheat landraces, susceptible control Zhengzhou 5389 and 36 resistance donor lines were tested for leaf rust resistance. The genotypes were tested in the greenhouse to 19 Puccinia triticina pathotypes for a seedling resistance, and in the field to a mixed pathotypes for slow rusting resistance in two cropping seasons (2016-2017 and 2017-2018) in Baoding, Hebei Province. Meanwhile, twelve molecular markers targeting the resistance loci were used for genotyping in wheat cultivars. Resulted from the resistance spectra and marker analysis, seven leaf rust resistance genes were detected in 15 landraces. For instance, the landrace Honggoudou may contain both Lr1 and Lr46; Huanghuamai may have both Lr13 and Lr34; Dabaimai may carry Lr14b and Lr26; Yangmai may carry Lr37 and Lr46; Chengduguangtou may carry Lr34 and Lr46. Two resistance genes Lr26 and Lr46 were found to be present in genotypes Motuomai and Xishanbiansui. Furthermore, the slowing rust resistance gene Lr34 was detected in Tongjiabaxiaomai, Wudubaijianer, Bianbachunmai-6 and Baihuamai, while another partial resistance gene Lr46 was detected in Hongqiangmai, Baibiansui and Baihuomai. These genotypes effective against leaf rust might provide resistance genes for disease-resistant breeding in hexaploid wheat.
Key words:  wheat landraces  leaf rust  gene postulation  molecular detection  slow rusting cultivar

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