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  • 韩雯毓,李国瑞,风 兰,等.蓖麻GRAS转录因子家族的全基因组分析及逆境胁迫响应[J].植物遗传资源学报,2020,21(1):252-259.    [点击复制]
  • HAN Wen-yu,LI Guo-rui,FENG Lan,et al.Genome-wide Analysis of GRAS Transcription Factors in Ricinus communis and Response to Abiotic Stresses[J].植物遗传资源学报,2020,21(1):252-259.   [点击复制]
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蓖麻GRAS转录因子家族的全基因组分析及逆境胁迫响应
韩雯毓, 李国瑞, 风 兰, 李孟建, 白英俊, 孙佳欣, 闫星伊, 王双, 赵秀萍, 陈永胜
0
(内蒙古民族大学)
摘要:
GRAS 转录因子在植物生长发育及逆境胁迫等多个生理生化途径中具有重要作用。本研究从蓖麻基因组中鉴定出48 个 GRAS 转录因子,对其理化性质、系统发育模式、基因结构与保守基序进行了分析。结果表明,蓖麻 GRAS 基因编码的蛋白全部为亲水蛋白,其中酸性蛋白质约占 94 %,等电点在 4.82-10.21 之间,相对分子量介于 17305.2-90986.6 之间。GRAS转录因子家族成员分为 11 个亚家族,同一亚家族成员具有相似的基因结构与保守基序。利用 RT-qPCR 技术检测了根、茎、叶不同组织中的五个基因在干旱与盐胁迫下的表达情况,结果表明,RcGRASs 在不同组织中的表达具有特异性,干旱与盐胁迫诱导 RcGRAS14、RcGRAS21、RcGRAS35 的表达,抑制 RcGRAS1、RcGRAS10 的表达。本研究为进一步研究 GRAS 转录因子在非生物胁迫中的功能提供参考。
关键词:  蓖麻  GRAS转录因子  生物信息学分析  逆境胁迫
DOI:10.13430/j.cnki.jpgr.20190521002
投稿时间:2019-05-21修订日期:2019-06-23
基金项目:国家自然科学基金项目(31460353);内蒙古自治区蓖麻育种重点实验室开放基金(MDK2018017,MDK2018018);内蒙古民族大学自治区科 技储备项目(2018NDCB05)
Genome-wide Analysis of GRAS Transcription Factors in Ricinus communis and Response to Abiotic Stresses
HAN Wen-yu, LI Guo-rui, FENG Lan, LI Meng-jian, BAI Ying-jun, SUN Jia-xin, YAN Xing-yi, WANG Shuang, ZHAO Xiu-ping, CHEN Yong-sheng
(Inner Mongolia University for Nationalities)
Abstract:
GRAS transcription factors play important roles in many physiological and biochemical pathways involved in plant growth and development and stress responses. 48 GRAS transcription factors were identified in the genome of Ricinus communis L., and their physical and chemical properties, phylogenetic patterns, gene structure, and conserved motifs were analyzed. The proteins encoded by GRAS genes of castor are all hydrophilic, with acidic proteins accounting for about 94%, isoelectric points between 4.82-10.21, and relative molecular weights between 17305.2-90986.6. The GRAS transcription factors are divided into 11 subfamilies,and members of the same subfamily have similar gene structures and conserved motifs. RT-qPCR was used to detect the expression of five genes in roots, stems and leaves under drought and salt stresses. The results showed that the expression of RcGRASs in different tissues was specific, and drought and salt stresses induced the expression of RcGRAS14、RcGRAS21 and RcGRAS35 but inhibited the expression of RcGRAS1 and RcGRAS10. This study provides a reference for further study of the functionon of GRAS transcription factors in abiotic stresses.
Key words:  castor (Ricinus communis L.)  GRAS transcription factors  bioinformatic analysis  abiotic stresses

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