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  • 李盼,李超,张显强,等.甘蓝型矮杆直立株型油菜株高性状基因的初步定位[J].植物遗传资源学报,2020,21(1):83-93.    [点击复制]
  • LI Pan,LI Chao,ZHANG Xian-qiang,et al.Low-resolution Mapping of a Qualitative Trait Controlling Plant Height in Brassica napus L[J].植物遗传资源学报,2020,21(1):83-93.   [点击复制]
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甘蓝型矮杆直立株型油菜株高性状基因的初步定位
李盼1, 李超2, 张显强3, 张瑞茂4, 罗京1
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(1.贵州师范大学生命科学学院;2.贵州省贵阳市花溪区贵州省农业科学院油菜研究所;3.贵州警察学院;4.贵州省农业科学院油菜研究所)
摘要:
定位甘蓝型矮秆直立株型油菜的株高基因,解析矮秆性状的遗传规律,对油菜育种的产量具有重要意义。通过甘蓝型三系油菜(不育系5824×恢复系5771R)杂交,在后代F1中发现变异单株,命名“DW871”。经多代自交,从群体中选取纯合矮秆和对应纯合高秆杂交,从杂交后代分离群体中分别选取47 个矮秆、47 个高秆和10 个纯合矮秆材料,分别构建基因池。利用BSA-seq简化基因组测序技术,以47 个矮秆与47 个高秆,10 个纯合矮秆与47 个高秆进行关联分析(BSA),从47 个矮秆与47 个高秆基因混合池间共检测到差异SNP共有121998 个,非同义突变SNP共1582 个,在ChrA10染色体上定位1个显著关联区域,区间长度6.39 Mb,含1405 个候选基因;从10 个纯合矮秆与47 个高秆基因混合池间共获得1752011 个SNP,非同义突变SNP共27723个,InDel 518420个,在ChrA03、ChrA04、ChrA06、ChrA07、ChrA10和ChrC03上定位共19 个与性状相关的候选区域,区间长度5.35 Mb,含1143个候选基因。然而由于未达到理论阈值,这个区域很可能是假阳性区域,需要进一步验证。47个矮秆基因混池和10个纯矮秆这2个基因混池在ChrA10染色体上定位的关联区域互相重合,重合区间为1.83 Mb。本研究在ChrA10染色体上定位1个与DW871株高性状显著关联区间,这一研究结果为精细定位甘蓝型矮秆直立株型油菜株高性状基因奠定良好基础。
关键词:  甘蓝型矮杆油菜  株高基因  BSA-seq技术
DOI:10.13430/j.cnki.jpgr.20190514001
投稿时间:2019-05-14修订日期:2019-11-20
基金项目:贵州省科学技术基金重点项目(黔科合基础[2017]1415);贵州省优秀青年科技人才项目(黔科合平台人才[2017]5642);贵州省支撑计划重点项目(黔科合支撑[2017]2568号);贵州省人才基地(RCJD2018-14);贵州植物保育技术应用省级工程研究中心
Low-resolution Mapping of a Qualitative Trait Controlling Plant Height in Brassica napus L
Abstract:
Genetic identification of the functional genes causing the erect dwarf-type are of great significance in academic study and rapeseed breeding. We previously identified a naturally-occurred dwarf mutant (named DW871) from a single F1 plant by crosses of sterile line 5824 × recovery line 5771R. By making crosses between the homozygous mutant with a homozygous wild-type line, 47 dwarf rods (DR), 47 high rods (HR) and 10 homozygous dwarf rod lines (HDR) were identified for a genetic mapping study. Three bulks were subjected for simplified genome sequencing technology (BSA-seq), in order to achieve the preliminary mapping of the underlying functional genes. A total of 121998 SNPs were detected between 47 dwarves and 47 high-stem mixed gene pools. 1582 SNPs resulting in non-synonymous mutations were located on ChrA10 chromosome, Interval length is 6.39 MB, including 1405 candidate genes . Out of 1752011 SNPs that were obtained between HR and HDR pools 27723 SNPs led to the non-synonymous mutations. A total of 19 candidate loci were mapped on ChrA03, ChrA04, ChrA06, ChrA07, ChrA10 and ChrC03, Interval length is 5.35 Mb ,including 1143 candidate genes. However, these locus showed un-significant associations and need to be further verified. Interestingly, the association region on the ChrA10 chromosome was revealed by cases and a coincidence interval of 1.83 Mb was suggested. As a summary, the qualitative locus controlling the plant height in DW871 was mapped on ChrA10 chromosome, and this result laid a good foundation for the future fine mapping of the functional gene in Brassica napus L.
Key words:  Brassica napus L  plant height gene  BSA-seq technology

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