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  • 陈珊宇,王大刚,郑桂杰,等.野生大豆对大豆花叶病毒株系SC13的抗性遗传和基因定位[J].植物遗传资源学报,2020,21(1):139-145.    [点击复制]
  • CHEN Shan-yu,WANG Da-gang,ZHENG Gui-jie,et al.Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC13 in Glycine soja[J].植物遗传资源学报,2020,21(1):139-145.   [点击复制]
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野生大豆对大豆花叶病毒株系SC13的抗性遗传和基因定位
陈珊宇1, 王大刚2, 郑桂杰2, 马莹2, 杨中路2, 曹栋栋1, 黄玉韬1, 智海剑2
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(1.浙江省农业科学院作物与核技术利用研究所;2.作物遗传与种质创新国家重点实验室/南京农业大学大豆研究所/国家大豆改良中心)
摘要:
大豆花叶病毒(Soybean mosaic virus,SMV)病是广泛分布于我国各大豆产区的大豆主要病害之一。SMV 株系SC13 是我国北方大豆产区广泛分布的株系之一。为拓宽大豆对 SMV 的抗病种质,本文研究了中国大豆核心种质材料野生大豆 ZYD03715 对大豆花叶病毒株系 SC13 的抗性遗传方式,确定与栽培大豆抗源对同一株系的抗性位点间的等位性关系,并对抗性基因进行了标记定位。结果表明:野生大豆抗源 ZYD03715 对 SMV 株系 SC13 的抗性由 1 对隐性基因控制,广谱抗源‘科丰 1 号’的抗性受 1 对显性基因控制,且两个抗源携带的抗性基因是不等位的。采用分离群体组群分析发现,野生大豆ZYD03715 对 SC13 的抗性位点(rySC13)位于大豆 14 号染色体(B2 连锁群)上,处于 2 个 SSR 标记 Satt416 和 Satt083 一侧,与其距离分别为 4.1 cM 和 0.9 cM。利用‘科丰 1号’ב南农 1138-2’的 F2群体,将‘科丰 1 号’所携带的抗性基因(RkSC13)定位在大豆 2 号染色体(D1b 连锁群)上的 Satt558 和 Sat_254 标记之间,遗传距离为 3.7cM 和 16.1 cM。以往发现大豆对 SMV不同株系的抗性都分别由一对显性基因控制,本研究在野生大豆中鉴定出隐性抗病基因,并标记定位了该隐性抗病基因,它将为大豆抗病性育种的分子标记辅助选择以及抗性基因的精细定位和克隆奠定基础。
关键词:  野生大豆  大豆花叶病毒  抗性  隐性  基因定位
DOI:10.13430/j.cnki.jpgr.20190513001
投稿时间:2019-05-13修订日期:2019-11-12
基金项目:国家重点研发计划(2017YFD0101501);国家自然科学基金(31571687, 31571690);中央高校基本科研业务费专项资金资助(KYT201801);长江学者和创新团队发展计划资助(PCSIRT_17R55);国家大豆产业技术体系(CARS-04);浙江省重点研发计划(2019C02004);江苏省现代作物生产协同创新(JCIC-MCP);安徽省自然科学基金(1708085MC69)
Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC13 in Glycine soja
CHEN Shan-yu1, WANG Da-gang2, ZHENG Gui-jie2, MA Ying2, YANG Zhong-lu2, CAO Dong-dong1, HUANG Yu-tao1, ZHI Hai-jian2
(1.Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences;2.National Key Laboratory for Crop Genetics and Germplasm Enhancement/Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement)
Abstract:
Soybean mosaic virus (SMV) disease is one of the major soybean diseases widely distributed in soybean production areas in China. SMV strain SC13 is among the major strains occurring in northern China. In order to broaden the germplasm of soybean resistant to SMV, the inheritance mode of resistance of wild soybean ZYD03715, a core germplasm material of soybean in China, against SMV strain SC13 was studied. The allelic relationship between the resistant germplasm of cultivated soybean and the resistance loci against the same strain was determined, and the resistance genes were marked and mapped. The results showed that the resistance of the wild soybean ZYD03715 against SMV strain SC13 was controlled by a pair of recessive genes, whereas the resistance of the broad-spectrum resistant germplasm ‘Kefeng 1 Hao’ was controlled by a pair of dominant genes, and the resistance genes carried by the two resistant germplasms were nonallelic. Through the bulked segregant analysis, the resistance loci (rySC13,) of the wild soybean ZYD03715 against SC13 were located on soybean chromosome 14 (B2 linkage group), on the side of two SSR markers Satt 416 and Satt 083, with genetic distances of 4.1 cM and 0.9 cM, respectively. The resistance gene ( RkSC13) carried by ‘Kefeng 1 Hao’ was mapped between Satt 558 and Sat_254 markers on soybean chromosome 2 (D1b linkage group) by using F2 population of ‘Kefeng 1 Hao’ × ‘Nannong 1138-2’, with genetic distances of 3.7 cM and 16.1 cM. In the past, it was found that the SMV-resistance of soybean was controlled by a pair of dominant genes. In this study, recessive resistance genes were identified in wild soybeanand marked and mapped. It will lay a foundation for molecular marker-assisted selection and fine mapping and cloning of resistance genes in soybean breeding for disease-resistance.
Key words:  Glycine soja  soybean mosaic virus  resistance inheritance  recessive  gene mapping

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