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水曲柳FmJAZ1基因在非生物胁迫中的响应模式和激素诱导表达分析
屈申, 刘宗林, 杨少彤, 于磊, 何利明, 韩丹宇, 詹亚光
0
(东北林业大学生命科学学院)
摘要:
克隆 FmJAZ1 基因,明确其在低温和 NaCl 胁迫中的响应模式和激素诱导下的转录表达特性。通过基因克隆的方法得到水曲柳中的 FmJAZ1 基因,利用生物信息学软件对所得到的序列进行分析并构建系统进化树,对水曲柳 FmJAZ1 基因进行了时空表达特异性的分析,对根,茎,叶,芽,雄花,雌花,种子七个部位,以及 5.6.7.8.9 五个月份分别取样,对水曲柳进行低温(4℃)和盐胁迫(NaCl 溶液)两种非生物胁迫处理和脱落酸(ABA),赤霉素(GA3),生长素(IAA),茉莉酸(JA)和水杨酸(SA)激素信号诱导处理,然后对实验材料进行荧光定量分析。克隆出全长为 684bp 的核苷酸序列。生物信息学软件分析得 JZA1 基因具有完整的开放阅读框,编码 227 个氨基酸,JAZ1 蛋白不含有信号肽,不属于跨膜蛋白,为不稳定亲水性蛋白;时空表达结果显示 FmJAZ1 基因在茎中表达量最高;FmJAZ1 基因在 8 月份表达量最高;非生物胁迫结果表明低温处理后 FmJAZ1 在 6h,24h 表达量较高;而 NaCl 处理后,在 24h 表达量较高,且该基因响应低温胁迫较盐胁迫迅速;激素信号诱导结果显示处理后不同时间,基因表达量变化较为明显,其中 GA3处理后 3h 最为明显,为对照组的 77.3 倍,分析了 FmJAZ1基因在低温、NaCl 胁迫和激素诱导下的表达模式。FmJAZ1 基因充分响应了逆境胁迫和激素信号诱导,通过蛋白和基因层面对逆境进行响应,JAZ 蛋白在其中起到了桥梁的作用,并扮演了重要的角色。
关键词:  FmJAZ1  非生物胁迫  激素信号诱导
DOI:10.13430/j.cnki.jpgr.20190507003
投稿时间:2019-05-07修订日期:2020-01-17
基金项目:国家重点研发计划课题(2017YFD0600605-01);大学生创新课题(201810225127)
Analysis of Expression of FmJAZ1 Gene in Fraxinus mandshurica: Response Model to Abiotic Stresses and Hormone Induction
QU Shen, LIU Zong-lin, YANG Shao-tong, YU Lei, HE Li-ming, HAN Dan-yu, ZHAN Ya-guang
(College of Life Science,Northeast Forestry University)
Abstract:
This study aims to clone the FmJZ1 gene and clarify its response model under low temperature and NaCl stresses and its hormone-induced transcriptional expression characteristics . The FmJAZ1 gene of Fraxinus mandshurica was obtained by gene cloning. The sequence was analyzed by bioinformatics software and phylogenetic tree was constructed.Spatiotemporal expression specificity of Fraxinus mandshurica FmJAZ1 gene was analyzed. Samples of root, stem, leaf, bud, male flower, female flower and seedwere taken in May, June, July, August, and September respectively for plants of Fraxinus mandshurica under low temperature stress (4℃) salt stress (NaCl solution), or treated with hormone signal induction of abscisic acid (ABA), gibberellin (GA3), auxin (IAA), jasmonate (JA), and salicylic acid (SA), The treated materials were then subjected to fluorescence quantitative analysis,The full length nucleotide sequence of 684 bp was cloned in this experiment, Bioinformatics software analysis showed that JZA1 gene had a complete open reading frame, encoding 227 amino acids. JAZ1 protein did not contain signal peptide, it was not a transmembrane protein, and it was an unstable hydrophilic protein. The spatial expression of FmJAZ1 gene was the highest in stem, and the temporal expression was the highest in August. The expression level of FmJAZ1 gene was higher at 6 h and 24 h after low temperature treatment. and higher 24 h after NaCl treatment, with the response to low temperature stress faster than that to salt stress. The expression level of FmJAZ1 gene changed significantly with time after the treatments hormone signal induction, and the expression pattern of FmJAZ1 gene was analyzed for low temperature, NaCl stress and hormone induction treatments. The expression change of FmJAZ1 gene was 77.3 times higher than that of control group at 3 h after GA3 treatment. FmJAZ1 gene fully responded to abiotic stresses and hormone signal induction, with JAZ protein acting as a bridge and playing an important role in the responses at protein and gene levels.
Key words:  FmJAZ1  abiotic stress  hormone signal induction

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