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山西小麦地方品种的初级核心种质构建
雷梦林1, 刘霞1, 李欣2, 张建华3, 王宇楠1, 曹利萍1, 张晋1, 黄蕊1, 穆志新4, 杜慧玲5, 冯瑞云2
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(1.山西省农业科学院农作物品种资源研究所/作物遗传与分子改良山西省重点实验室/农业部黄土高原作物基因资源与种质创制重点实验室/杂粮种质 资源发掘与遗传改良山西省重点实验室;2.山西省农业科学院作物科学研究所;3.山西省农业科学院小麦研究所;4.山西省农业科学院农作物品种资源研究所;5.山西农业大学农学院)
摘要:
以410份山西小麦地方品种为试验材料,根据田间农艺性状,采用取样比例、取样方法、遗传距离和系统聚类方法相结合的方法构建山西小麦地方品种的初级核心种质,确定了“多次聚类优先取样法+19.27%取样比例+马氏距离+中间距离法”的取样策略最佳,共79份材料入选山西小麦地方品种初级核心种质。应用均值差异百分率参数(MD)、方差差异百分率参数(VD)、极差符合率参数(CR)和变异系数变化率参数(VR)来评价初级核心种质的优劣,其MD、VD、CR和VR评价参数分别为0、69.57%、100.00%和133.93%。经初级核心种质和基础样本的遗传多样性指数的t值检验、符合率和主成分比较,发现初级核心种质和基础样本的总多样性指数相差仅0.07,核初级心种质和基础样本t值检验差异不显著、初级核心种质和基础样本符合率最高达100.00%、初级核心种质比基础样本主成分累计贡献率提高了6.49%,说明构建的初级核心种质能够代表基础样本的遗传多样性,为山西小麦地方品种资源的利用创新提供了优先参考群体。
关键词:  小麦  地方品种  农艺性状  核心种质  遗传多样性
DOI:10.13430/j.cnki.jpgr.20190429001
投稿时间:2019-04-29修订日期:2020-01-08
基金项目:山西省农业科学院特色农业技术攻关项目(YGG17059);山西省重点实验室运行补助(201705D11108-32);山西省重点科技创新平台项目 (201605D15100100.002)
Construction of Primary Core Collection of Wheat Landraces Based on Agronomic Traits in Shanxi Province
LEI Meng-lin1, LIU Xia1, LI Xin2, ZHANG Jian-hua3, WANG Yu-nan1, CAO Li-ping1, ZHANG Jin1, HUANG Rui1, Mu Zhixin4, DU Hui-ling5, FENG Rui-yun2
(1.Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Molecular Improvement of Shanxi Province / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture /Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops;2.Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences;3.Institute of Wheat, Shanxi Academy of Agricultural Sciences;4.Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Genetics and Molecular Improvement of Shanxi Province/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture /Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan;5.College of Agriculture, Shanxi Agricultural University)
Abstract:
410 wheat landraces from Shanxi Province were used as test materials for construction of the primary core collection of wheat landraces of the Province based on their agronomic traits in the field, with different combinations of sampling proportion, sampling method, genetic distance and clustering method. The combination of multiple cluster priority sampling method, 19.27% sampling proportion, Mahalanobis distance, and middle distance method was identified as the optimal sampling strategy, with which 79 wheat landraces were selected for inclusion in the primary core collection. The primary core collection was evaluated by MD (0), VD (69.57%), CR (100.00%) and VR (133.93%). By comparing the primary core collection and the total basic sample collection of genetic diversity index, t-value, accord rate and principal component, it was found that the primary core collection and the basic sample collection total diversity index differed by only 0.07. The t-value test showed no significant difference between the primary core collection and the basic sample collection, the primary core collection and the basic sample collection met the highest rate of 100.00%, and the cumulative contribution rate of primary core collection to principal component of the basic sample collection increased by 6.49%. The results showed that the constructed primary core collection could represent the genetic diversity of the basic sample collection and could be used as a priority reference population of the utilization and innovation of wheat landraces in Shanxi Province.
Key words:  wheat  landraces  agronomic traits  core collection  genetic diversity

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