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  • 刘渊,孔佑宾,李喜焕,等.基于SLAF-BSA技术挖掘大豆酸性磷酸酶活性候选基因挖掘及标记开发[J].植物遗传资源学报,2020,21(1):164-173.    [点击复制]
  • LIU Yuan,KONG You-bin,LI Xi-huan,et al.SLAF-BSA Assisted Mining for Candidate Genes with Acid Phosphatase Activity and Functional Marker Exploitation in Soybean[J].植物遗传资源学报,2020,21(1):164-173.   [点击复制]
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基于SLAF-BSA技术挖掘大豆酸性磷酸酶活性候选基因挖掘及标记开发
刘渊, 孔佑宾, 李喜焕, 张彩英
0
(河北农业大学农学院分子遗传育种实验室)
摘要:
磷利用效率与大豆产量密切相关,根尖酸性磷酸酶活性是筛选大豆品种磷效率的重要指标。挖掘酸性磷酸酶活性候选基因并开发其功能标记对获得磷高效功能基因、解析磷利用分子机制和培育磷高效大豆新品种意义重大。本研究利用酸性磷酸酶活性重组自交系群体F12构建了两个极端性状混池DNA文库,通过SLAF-BSA 技术,获得了268个与大豆酸性磷酸酶活性关联的SNP,包括12个非同义突变,其中亲本间7个,后代混池间5个;在2个关联候选区域,获得79个酸性磷酸酶活性相关基因,其中第3号染色体的20138271~20268154间4个,17号染色体的14368648~15526449间75个;对该区域内基因进行了功能注释。开发了非同义突变基因Glyma.17G166200.1功能标记GMsnp-B,用该标记检测169份大豆栽培品种基因型,与表型符合率达到82.8%。
关键词:  大豆  酸性磷酸酶  SLAF-BSA  候选基因  功能标记
DOI:10.13430/j.cnki.jpgr.20190428001
投稿时间:2019-04-28修订日期:2019-11-25
基金项目:现代农业科技奖励性后补助资金项目(17927670H);河北省科技计划项目(16227516D)
SLAF-BSA Assisted Mining for Candidate Genes with Acid Phosphatase Activity and Functional Marker Exploitation in Soybean
LIU Yuan, KONG You-bin, LI Xi-huan, ZHANG Cai-ying
(Laboratory of Molecular Genetics And Breeding,College of Agronomy, Hebei Agricultural University)
Abstract:
The activity of acid phosphatase in root tips is an important index for screening phosphorus efficiency of soybean varieties. Mining for candidate genes with acid phosphatase activity and exploiting their functional markers will bring great significance for identifying the phosphorus-efficient genes, unlocking the molecular mechanism of phosphorus utilization as well as breeding for new phosphorus-efficient soybean varieties. In this study, two DNA libraries with bulked pools of segregants from a F12 recombination inbred line population were constructed for next generation sequencing. 268 significant SNPs were obtained by SLAF-BSA technique. That included 12 gene-based non-synonymous mutations, seven of which were found between parents and five of which were found between offspring pools. Particularly, two candidate regions were identified, where 79 acid phosphatase-related genes were resided. Four genes are allocated from 20138271 to 20268154 on chromosome 3 and 75 genes are allocated from 1436648 to 15526449 on chromosome 17. Furthermore, a genetic marker GMsnp-B, which targets to the non-synonymous mutation gene Glyma.17G166200.1, was developed and tested using 169 soybean cultivars. The coincidence rate with phenotype activities in root tips was 82.8%.
Key words:  soybean  acid phosphatase  SLAF-BSA  candidate gene  functional marker

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