大豆UBC基因家族鉴定及GmUBC46基因的功能初步分析
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哈尔滨师范大学生命科学与技术学院

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黑龙江省自然科学基金面上项目(C2017039);黑龙江省教育厅面上项目(12531178)


Identification of Soybean UBC Gene Family and Preliminary Functional Analysis of GmUBC46
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School of Life Science and Technology, Harbin Normal University

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Heilongjiang Provincial Natural Science Foundation Project (C2017039) and Heilongjiang Provincial Education Department Project (12531178)

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    摘要:

    泛素/26S 蛋白酶体途径在植物响应非生物胁迫反应中起着重要的作用。E2(泛素结合酶,UBC)是蛋白质泛素化中 重要的泛素结合酶,与 E1 和 E3 共同参与蛋白降解途径。本研究通过构建隐马尔可夫模型,鉴定了 54 个大豆 UBC 基因,通过 进化树分析将该家族成员分为 11 个亚家族(A-K)。蛋白保守结构域分析表明,GmUBC 家族蛋白均含有保守 Motif 1、2 与 3, 且均属于泛素结合酶保守结构域。组织定位分析表明 GmUBC 家族基因在大豆根、茎、叶、花等组织中均有所表达。转录组数据 表明有 20 个 GmUBC 基因在干旱、盐或冷胁迫下具有不同的表达模式,启动子顺式作用元件分析发现其胁迫响应过程可能与激 素信号转导相关。进一步通过 qRT-PCR 发现 GmUBC46 基因能积极响应干旱、盐或冷胁迫诱导上调表达。通过酵母功能验证表 明,GmUBC46 基因降低了对干旱或盐胁迫的耐受性。综上,本研究初步阐明了大豆 UBC 基因家族的基本特性及 GmUBC46 基 因的耐逆功能,为后续研究提供了重要依据和参考价值。

    Abstract:

    The ubiquitin/26S proteasome pathway plays an important role in response to abiotic stress in plants. E2 (ubiquitin-binding enzyme, UBC), which is an ubiquitin-binding enzyme involved in protein ubiquitination, participates in the protein degradation pathway in a complex with E1 and E3. In this study, we identified 54 soybean UBC genes using hidden Markov model, and the family members were divided into 8 subfamilies (A-K) according to the phylogenetic tree analysis. Protein conserved domain analysis indicated that GmUBC family proteins contain conserved domains 1, 2 and 3, which belong to the conserved domain of ubiquitin-binding enzymes. Tissue expression analysis indicated that the transcripts of GmUBC family genes were detected in soybean roots, stems, leaves, flowers and other tissues. Transcriptome data indicated that a diversifying expressional pattern was observed for 20 GmUBC genes under drought, salt or cold stress treatments. Analysis for cis-elements in the promoter suggests that some of GmUBC genes may be involved in hormonal signaling. Furthermore, qRT-PCR analysis showed that GmUBC46 was inducible under drought, salt and cold stresses. Expressing the GmUBC46 gene in yeast showed a decreased tolerance in responses to drought and salt stress treatments. In summary, this study revealed the basic characteristics of UBC gene family in soybean and the preliminary functional insight of GmUBC46, and gain of datasets might provide considerable basis and reference value for future research.

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陈超,乔艳花,井常煦,等.大豆UBC基因家族鉴定及GmUBC46基因的功能初步分析[J].植物遗传资源学报,2020,21(1):154-163.

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  • 收稿日期:2019-04-23
  • 最后修改日期:2019-05-26
  • 录用日期:2019-07-22
  • 在线发布日期: 2020-01-17
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