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来自西尔斯山羊草的抗小麦白粉病基因Pm57抗性丧失突变体的筛选与鉴定
马超, 董振杰, 田修斌, 王贝麟, 张玥琦, 李欢欢, 刘文轩
0
(河南农业大学生命科学学院)
摘要:
亲缘种属来源的抗白粉病基因是培育小麦抗病品种,防治白粉病危害的最重要基因来源。Pm57是位于西尔斯山羊草 2Ss#l 染色体长臂上的一个外源基因,对小麦白粉病具有苗期和成株期广谱抗性。为了创制 Pm57 白粉病抗性丧失突变体,利用基于基因突变体的植物抗病基因克隆新兴技术分离 Pm57 基因,我们选用 0.625%的甲基磺酸乙酯(EMS)对 1 万粒小麦-西尔斯山羊草 Pm57 易位系 89(5)69 种子进行了诱变处理,M1 大田密播种植,收获了 1598 个 M2 可育株系。初步对其中 300 个 M2株系进行苗期白粉病抗性接种鉴定,并利用 2 个 Pm57 基因特异分子标记 X2L4g9P4/HaeIII 和 X284274 及小麦全国区试品系 DUS 测试所用的 42 对 SSR 核心引物对 Pm57 抗性丧失突变体进行鉴定,筛选出来自 27 个 M2株系的真实抗性丧失突变体 70 个,Pm57 基因抗性丧失突变体频率达到 9.0%。本研究所获得的白粉病抗性丧失突变体为 Pm57 基因的后续克隆与抗白粉病分子机理研究提供了重要的材料基础。
关键词:  小麦白粉病  Pm57  甲基磺酸乙酯  抗性丧失突变体  分子标记
DOI:10.13430/j.cnki.jpgr.20190417001
投稿时间:2019-04-17修订日期:2019-10-25
基金项目:国家自然科学基金项目(31571658);河南省科技重大专项(161100110400),
Screening and Identification of Susceptible Pm57 Mutants, Whose Modifications Compromise to Powdery Mildew Derived from Aegilops searsii
MA Chao, DONG Zhen-jie, TIAN Xiu-bin, WANG Bei-lin, ZHANG Yue-qi, LI Huan-huan, LIU Wen-xuan
(College of Life Sciences, Henan Agricultural University)
Abstract:
Wheat relatives-derived alien genes provide most important resource to develop resistant varieties and protect wheat from the damage of powdery mildew. Pm57, a gene resided on the long arm of chromosome 2Ss#1 from Aegilops searsii caused broad-spectrum resistance against powdery mildew isolates at seedling and adult plant stage. In order to develop Pm57 mutants with loss of resistance to powdery mildew for further gene isolation using susceptible mutant-based resistant cloning approache, 10000 seeds of 89(5)69, a wheat-Ae. searsii recombinant stock containing Pm57 genes,were treated using 0.625% Ethyl methane sulfonate (EMS). M1seeds were then dense sowed in experiment station, and 1598 M2 lines were harvested. Of which 300 M2 lines randomly selected were used for resistance test by inoculation at seedling stage. After further validation of susceptible individual plants using two Pm57 specific molecular markers (X2L4g9P4/HaeIII and X284274) and 42 pairs of core primers,which are applied in DUS identification of wheat lines, 70 Pm57 mutants with loss of resistance to powdery mildew from 27 independent M2 lines were identified, with a mutant ratio as high as 9.0%. Thus, the newly-identified resistance-compromised mutants might lay a foundation for further cloning of the Pm57 gene.
Key words:  wheat powdery mildew  Pm57  ethyl methane sulfonate  mutant  molecular markers

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