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国外引进水稻种质资源的稻瘟病抗性基因检测与评价
朱业宝, 方珊茹, 沈伟峰, 陈立喆, 江川, 王金英
0
(福建省农业科学院水稻研究所)
摘要:
为了筛选出福建省水稻稻瘟病重发区育种中可利用的新抗性资源,在福建省上杭县对 156 份外引水稻种质资源进 行了 2 年田间自然诱发鉴定,并对 Pi2、Pi9、Pi5、Pi54、Pikm、Pita、Pia 和 Pib 等 8 个稻瘟病抗性基因做了分子检测。结 果表明:156 份资源对苗瘟、叶瘟、穗颈瘟和综合抗性表现抗病的分别有 10 份、14 份、29 份和 26 份,且苗瘟抗性级别与叶 瘟抗性级别(r=0.816,P<0.01)、苗瘟抗性级别与穗颈瘟抗性级别(r=0.347,P<0.01)、以及叶瘟抗性级别与穗颈瘟抗性级别 (r=0.344,P<0.01),均呈极显著正相关。分子标记检测到携带稻瘟病抗性基因 Pi9、Pi2、Pi54、Pikm、Pi5、Pib、Pia 和 Pita 的水稻资源分别有 1、6、20、22、37、88、101 和 106 份,其中携带稻瘟病抗性基因 Pi9 和 Pi2 的水稻资源的抗性表现较好, 表现抗病的超过 60%, 携带其他稻瘟病抗性基因的水稻资源表现抗病的均在 50%以下;水稻资源携带 0~6 个稻瘟病抗性基因, 随着携带抗性基因数目增加,抗病率呈上升趋势,综合抗性等级呈下降趋势。进一步研究发现,携带 Pi9+Pi5+Pikm+Pia、 Pi5+Pib+Pita+Pikm+Pia 和 Pi2+Pi54+Pib+Pita+Pikm+Pia 等 3 个基因型的水稻资源,稻瘟病抗性较好。最后,筛选了 8 份 稻瘟病抗性较好的材料,提供育种者参考、利用。
关键词:  稻种资源  稻瘟病  基因检测  抗性鉴定
DOI:10.13430/j.cnki.jpgr.20190414003
投稿时间:2019-04-14修订日期:2019-07-25
基金项目:福建省公益类科研院所基本科研专项(2017R1021-4);福建省重点农业县农作物种质资源的调查收集及其评价与应用(A2017-8);福建省自然科学基金项目(2016J01134);福建省农业科学院科技创新团队项目(STIT2017-1-1 );物种品种资源保护费项目(11821301354052031)
Detection and Evaluation of Blast Resistance Genes in Exotic Rice Germplasm Resources
ZHU Ye-bao, FANG Shan-ru, SHEN Wei-feng, CHEN Li-zhe, JIANG Chuan, WANG Jin-ying
(Rice Research Institute, Fujian Academy of Agricultural Sciences)
Abstract:
In order to identify new resistant resources of 156 exotic rice germplasm accessions for breeding in Fujian Province, test for rice blast resistance was conducted at two calendar years in experimental plot in Shanghang, Fujian. Genotypes carrying any of rice blast resistance genes Pi2, Pi9, Pi5, Pi54, Pikm, Pita, Pia and Pib were detected by molecular selection markers. As a result, 10, 14, 29 and 26 accessions showed resistant tseedling blast, leaf blast, neck blast and comprehensive resistance, respectively. Furthermore, the correlation analysis suggested significant positive correlations between seedling blast and leaf blast (r = 0.816, P < 0.01), seedling blast and neck blast (r = 0.347, P < 0.01), and leaf blast and neck blast (r = 0.344, P < 0.01). Marker assisted selection indicated 1, 6, 20, 22, 37, 88, 101 and 106 rice accessions, which harbored the resistance genes Pi9, Pi2, Pi54, Pikm, Pi5, Pib, Pia and Pita, respectively. 60% of accessions containing either of Pi9 and Pi2 genes were resistant to rice blast. A subset of rice accessions was found with stacking of 0 to 6 blast resistance genes. An elevation on rice blast resistance was observed if several resistance genes were present in an accession. For example, a combination of resistance genes Pi9+Pi5+Pikm+Pia, Pi5+Pib+Pita+Pikm+Pia and Pi2+Pi54+Pib+Pita+Pikm+Pia displayed the better resistance. Finally, 8 rice blast resistant genotypes were identified with potential for rice breeding.
Key words:  rice germplasm resources  rice blast  gene detection  resistance identification

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