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  • 赵陆滟,曹绍玉,龙云树,等.全长转录组测序在植物中的应用研究进展[J].植物遗传资源学报,2019,20(6):1390-1398.    [点击复制]
  • ZHAO Lu-yan,CAO Shao-yu,LONG Yun-shu,et al.Applications and Research Progresses of Full-length Transcriptome Sequencing in Plants[J].植物遗传资源学报,2019,20(6):1390-1398.   [点击复制]
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全长转录组测序在植物中的应用研究进展
赵陆滟, 曹绍玉, 龙云树, 张应华, 许俊强
0
(云南农业大学 云南省滇台特色农业产业化工程研究中心)
摘要:
全长转录组测序(Iso-Seq)是指使用第三代测序(TGS)技术获得mRNA的全长序列。随着以单分子实时(SMRT)测序为代表的TGS技术逐渐发展成熟,以及该技术在基因组和转录组de novo测序等方面的优势,全长转录组测序技术已经广泛被应用于动物、植物、微生物测序研究中,并挖掘出大量相关数据。本文综述了基于Iso-Seq技术的原理及其在模式植物和非模式植物研究中的应用,期望为研究植物全长转录组相关分析提供参考。
关键词:  全长转录组测序  Iso-Seq  TGS  单分子实时测序  模式植物和非模式植物
DOI:10.13430/j.cnki.jpgr.20190326001
投稿时间:2019-03-26修订日期:2019-04-30
基金项目:国家自然科学基金(31560560);云南省应用基础研究基金(2015FD019)
Applications and Research Progresses of Full-length Transcriptome Sequencing in Plants
ZHAO Lu-yan, CAO Shao-yu, LONG Yun-shu, ZHANG Ying-hua, XU Jun-qiang
(Dian-Tai Engineering Research Center for Characteristic Agriculture Industrialization of Yunnan Province, Yunnan Agricultural University)
Abstract:
Full-length transcriptome sequencing (Iso-Seq) is a technique method by deployment of the third generation sequencing (TGS) technology to obtain the full length sequence of mRNA. With the development of TGS technology which is represented by single molecule real-time (SMRT) sequencing, is becoming more and more mature, and it’s application advantages in genome and transcript de novo sequencing, full-length transcriptome sequencing technology has been widely used in animals, plants and microorganisms, and produced a large number of related data. In this paper, the principle of Iso-Seq technology and the applications of full-length transcriptome sequencing in model and non-model plants are reviewed, which aims to provide reference in analysis of full-length transcripts in plants.
Key words:  full-length transcriptome sequencing, Iso-Seq, TGS, single-molecule real-time sequencing, model and non-model plants

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