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  • 高斌,陈娟娟,崔顺立,等.花生bZIP基因家族全基因组鉴定及抗旱表达分析[J].植物遗传资源学报,2020,21(1):174-191.    [点击复制]
  • GAO Bin,CHEN Juan-juan,CUI Shun-li,et al.Genome-wide Identification and Expression Analysis of bZIP Gene Family under Drought Stress in Peanut[J].植物遗传资源学报,2020,21(1):174-191.   [点击复制]
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花生bZIP基因家族全基因组鉴定及抗旱表达分析
高斌, 陈娟娟, 崔顺立, 侯名语, 穆国俊, 陈焕英, 杨鑫雷, 刘立峰
0
(华北作物种质资源研究与利用教育部重点实验室/河北省种质资源实验室/河北农业大学农学院)
摘要:
本研究利用野生二倍体花生基因组信息鉴定出 112 个 bZIP 转录因子家族成员,其中 AA 基因组中 55 个家族成员,BB 基因组中 57 个家族成员,分别被命名为 AradubZIP1-AradubZIP55 与 AraipbZIP1-AraipbZIP57。通过生物信息学方法,分析了其基因结构、保守基序、理化性质,研究了其与拟南芥 bZIP 家族成员和部分四倍体花生 bZIP 的系统进化关系,预测了其在植物细胞中的位置,并利用转录组测序技术探究了部分家族成员在栽培种花生 L422 生长后期叶片响应干旱胁迫的基因表达规律。结果表明:野生二倍体中 bZIP 成员分布于 AA 和 BB 基因组的 10 条染色体上,均为不稳定蛋白,大多数定位在细胞核内(AA 基因组 36 个、BB 基因组 39 个),少数定位在叶绿体(14 个与 15 个)和线粒体(4 个与 3 个);具有相似基因结构与基序 bZIP 成员各 25 个,然而,不同成员间存在外显子数目差异,最多为 15 个外显子(AraipbZIP5),最少 1个(AraipbZIP1 与 AradubZIP10 等 12 个成员);通过与拟南芥 bZIPs 氨基酸序列进化分析发现,花生 bZIP 家族成员划分为A-M、S 等 14 个亚组,其中四倍体 bZIP 成员在第 I 亚组中分布最多(7 个);32 个四倍体 bZIP 成员在干旱胁迫下的表达模式基本分为 4 类,Ⅰ)前、后期高,中期低,Ⅱ)前期低,中、后期高,Ⅲ)前、中期高,后期低,Ⅳ)前、后期低,中期高。上述结果为研究花生 bZIP 基因家族在生长后期耐旱生长过程的调控作用提供参考依据。
关键词:  花生  bZIP转录因子  生物信息学分析  干旱胁迫  基因表达
DOI:10.13430/j.cnki.jpgr.20190324001
投稿时间:2019-03-24修订日期:2019-06-03
基金项目:国家现代农业产业技术体系建设项目(CARS-13); 国家自然科学基金(31701459); 河北省自然科学基金(C2017204101); 河北省科技计划项目(16226301D) ; 河北省现代农业产业技术体系油料创新团队项目(HBCT2018090202)
Genome-wide Identification and Expression Analysis of bZIP Gene Family under Drought Stress in Peanut
GAO Bin, CHEN Juan-juan, CUI Shun-li, HOU Ming-yu, MU Guo-jun, CHEN Huan-ying, YANG Xin-lei, LIU Li-feng
(North China Key Laboratory for Crop Germplasm Resources of Education Ministry / Laboratory for Crop Germplasm Resources of Hebei / College of Agronomy, Hebei Agricultural University)
Abstract:
In the present study, 112 bZIP transcription factors were identified from the reference genome of wild diploid peanut, including 55 and 57 family members on AA and BB genome, respectively. All the members were named AradubZIP1-AradubZIP55 and AraipbZIP1-AraipbZIP57. The gene structure, conservative motif, physiochemical properties were predicted through bioinformatics methods. Furthermore, the phylogenetic relationship of bZIP family members was analyzed between peanuts and Arabidopsis, and the subcellular localization was predicted in plant cells. To understand the biological function of these genes under drought stress, the transcriptional profiles of thirty-two members were analyzed by use of the transcriptome sequencing data of peanuts leaves at the late growth stage in a tetraploid peanut L422. The results indicated that the bZIP members in wild diploids were assigned into 10 chromosomes on AA and BB genomes, respectively. Most of them were localized to the nucleus (36 members in AA genome, 39 members in BB genome), and few of them were localized to chloroplasts (14 and 15) and mitochondria (4 and 3). Meanwhile, 25 members with similar gene structure/motif were found on two diploid peanut genome, respectively. The number of exons were variable, for instance that up to 15 exons was found in AraipbZIP5, and one exon was found in 12 bZIP genes. Furthermore, the peanut bZIPs were divided into 14 subgroups (A-M and S). Among these, seven members of tetraploid bZIPs were classified into the I subgroup. Finally, the transcriptional profiles of 32 tetraploid bZIP genes under drought stress treatment were divided into 4 categories, I) the early and late period were higher than the medium period, II) the middle and late period were higher than the early period, III) the early and medium period were higher than the late period, and Ⅳ) the middle period was higher than the early and late period. These results provided a useful reference for understanding the function of bZIP gene family members under the drought stress treatment at the late growth stage in peanut.
Key words:  peanut  bZIP transcription factor  bioinformatics analysis  drought stress  gene expression

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