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陆地棉EMS诱变体系的优化及种子和纤维发育异常突变体的鉴定
陈煜, 霍雪寒, 宋章强, 张传云, 杜召海, 周娟, 张景霞, 王芙蓉, 张军
0
(农业部黄淮海棉花遗传改良与栽培生理重点实验室/山东棉花研究中心,济南 250100)
摘要:
突变体是进行基因功能研究的遗传学材料。本研究利用浓度为0 ~ 1.5% EMS 溶液处理已萌发露白的鲁6269 种子4 ~ 12 h,分析不同浓度的EMS 溶液处理不同时间对棉花出苗率和生长的影响,并在M2 和M3 代对突变体进行了初步的形态学 鉴定。结果表明,1.0% EMS 溶液处理10h,鲁6269 的出苗率为49%(用原始记录的数据),为该材料的半致死浓度,诱变效 果最佳。为了获得大量的突变体,利用该浓度,处理鲁6269 萌发的种子(约10000 粒)10h,在M2 代发现一株纤维发育异 常(纤维成熟脱水后不蓬松)的突变体,收获该突变体自交种子,M3 代跟踪调查,发现该突变体纤维发育异常表型与M2 代 相同,种子解剖观察发现,该突变体种胚与外种皮之间存在一个大空腔,种胚弱小且扁瘪,影响种子的出土。本研究优化建 立了陆地棉品种鲁6269 种子EMS 诱变体系,在M3 代筛选获得一个种子与纤维发育异常的突变体sfm-1,为研究棉花种子及 纤维发育提供了遗传学材料。
关键词:  甲基磺酸乙酯(EMS)  突变体  种子  纤维发育  鲁6269
DOI:10.13430/j.cnki.jpgr.20181025002
投稿时间:2018-10-25修订日期:2018-11-07
基金项目:山东省农科院青年基金(2016YQN06);国家自然基金(31601345和 31671742)
Optimization of EMS Mutagenesis Condition in Upland Cotton and Screening of Mutant for Abnormal Seed and Fiber Development
CHEN Yu, HUO Xue-han, SONG Zhang-qiang, ZHANG Chuan-yun, DU Zhao-hai, ZHOU Juan, ZHANG Jing-xia, WANG Fu-rong, ZHANG Jun
(Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture/Cotton Research Center of Shandong Academy of Agricultural Sciences, Jinan 250100)
Abstract:
Mutants are excellent genetic materials for revealing molecular mechanisms of important genes. In this research, the cotton seeds were mutagenized by different concentrations of EMS solution (0%, 0.2%, 0.5%, 1.0% and 1.5%) and processing duration (4 h, 8 h, 10 h and 12 h), respectively, in order to optimize the reaction condition for EMS mutagenesis in upland cotton. Then, the effect of different EMS concentrations and treatment duration on germination rate and growth of sprouted cotton seeds was analyzed and the morphological characters of M2 and M3 generation were identified. The results showed that 1.0% EMS for 10 h was optimal for Lu6269 mutagenesis. The seed germination rate was about 49%, and the half lethal concentration is 1.0%. To obtain abundant mutants, approximated 10000 seeds of Lu6269 were treated with 1.0% EMS for 10h. A fiber abnormal mutant (the fiber non-fluffy in the development of maturation, sfm-1) was found in the M2 generation, and this phenotype was inherited by observing M3 generation seeds. This mutant showed a large cavity between the embryo and the seed coat, a weak and small embryo as well as decreased unearthing of seeds. Taken together, by establishing the EMS mutagenesis system of G. hirsutum cv Lu6269, we obtained sfm-1, a stable mutant for abnormal seed and fiber development, which served as the material foundation for fiber development, seed germination and function genome research in cotton.
Key words:  ethyl methanesulfonate (EMS)  mutant  seed size  fiber development  Lu6269

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