引用本文
  • 王焱,沙柏平,李明雨,等.苜蓿种质资源萌发期抗旱指标筛选及抗旱性综合评价[J].植物遗传资源学报,2019,20(3):598-609.    [点击复制]
  • WANG Yan,SHA Bai-ping,LI Ming-yu,et al.Indices Screening and Comprehensive Evaluation of Drought Resistance in Alfalfa Germplasm Resources at Germinating Stage[J].植物遗传资源学报,2019,20(3):598-609.   [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 1347次   下载 1414 本文二维码信息
码上扫一扫!
苜蓿种质资源萌发期抗旱指标筛选及抗旱性综合评价
王焱1, 沙柏平1, 李明雨1, 李雪1, 高雪芹1,2, 伏兵哲1,3
0
(1宁夏大学农学院,银川 750021;2西北土地退化与重建教育部重点实验室,宁夏银川 750021;3宁夏优势特色作物现代分子育种重点实验室,银川 750021)
摘要:
为筛选苜蓿萌发期抗旱性鉴定指标以及抗旱苜蓿种质材料,为苜蓿抗旱新品种选育提供理论和基础材料。以59份抗旱性不同的国内外苜蓿种质为材料,设置加蒸馏水和加-0.6MpaPEG-6000水溶液2个处理,研究了与抗旱性相关的根长、芽长、发芽势、发芽率、发芽指数、活力指数和根芽比等7个指标的变化,采用相关性分析、隶属函数、综合抗旱系数、灰色关联、逐步回归和聚类分析相结合的方法,对59份苜蓿种质萌发期抗旱性进行综合评价及抗旱指标筛选。结果表明:干旱胁迫对苜蓿萌发期各指标均有显著影响。筛选出萌发期抗旱性较强的苜蓿种质材料有草原3号、赛迪7、WL903,可作为抗旱育种和抗旱机理研究材料。根长、发芽率和活力指数对干旱胁迫的反应较其他指标敏感,可作为苜蓿品种抗旱性鉴定及抗旱品种选育时优先考虑的指标。试验结果也说明采用以D值为主要参数,以WDC值作为辅助参数的综合评价方法进行苜蓿抗旱性综合评价、评价指标筛选是合理准确的。
关键词:  苜蓿  萌发期  抗旱性  综合评价
DOI:10.13430/j.cnki.jpgr.20180901001
投稿时间:2018-09-01修订日期:2019-03-16
基金项目:宁夏牧草育种专项(2014NYYZ04);宁夏高等学校一流学科建设(草学学科)资助项目(NXYLXK2017A01)
Indices Screening and Comprehensive Evaluation of Drought Resistance in Alfalfa Germplasm Resources at Germinating Stage
WANG Yan1, SHA Bai-ping1, LI Ming-yu1, LI Xue1, GAO Xue-qin1,2, FU Bing-zhe1,3
(1Agricultural College, Ningxia University, Yinchuan 750021;2Key Laboratory of Northwest Land Degradation and Reconstruction, Ministry of Education, Yinchuan, Ningxia 750021;3Key Laboratory of Modern Molecular Breeding of Superior Characteristic Crops in Ningxia, Yinchuan 750021)
Abstract:
In order to screen the drought resistance identification index and the drought-resistant alfalfa germplasm materials, and provide the theory and basic materials for the breeding of new drought-resistant varieties. In this study, we investigated the changes of root length, bud length, germination potential, germination rate, germination index, vitality index and root bud ratio of 59 alfalfa germplasms with different drought resistance that are from different region under drought stress condition, which was stimulated by using two treatments including distilled water and -0.6MPa PEG-6000 water solution. Correlation analysis, subordinate function coefficients, comprehensive drought resistance coefficient, grey relational analysis, stepwise regression and clustering analysis were used to evaluate the drought resistance and select evaluation indices in tested alfalfa cultivars during the germination period. The results showed that drought stress had significant effects on all indexes at the germination stage. By employment of this approach, we obtained three alfalfa genotypes, including Caoyuan NO.3, Sidey7 and WL903, which showed strong drought-resistant at the germination stage. These germplasms were selected as the research material for drought resistance breeding and drought resistance mechanism. Notably, root length, germination rate and vitality index were more sensitive than other indicators and could be used as a priority indicator for drought resistance identification and breeding of drought-resistant varieties. The results also showed that it is reasonable and accurate to use the comprehensive evaluation method with D value as the main index and WDC value as the auxiliary index in alfalfa to carry out comprehensive evaluation of drought resistance and screening of evaluation indicators.
Key words:  alfalfa germplasms  germination stage  drought resistance  comprehensive evaluation

用微信扫一扫

用微信扫一扫