Broomcorn millet (Panicum miliaceum L.) is one of the minor crops in China, which represented resistant and tolerant against drought, lower-fertilizer and abiotic stresses as well as short life-cycle. Within this study, by generating an EMS-triggered mutant population by using broomcorn millet variety Yi-Xuan-Da-Hong-Mi, we analyzed the mutation frequency and phenotypic variation of 2333 M2 plants. Out of that, a total of 103 plants with visible phenotype differing with the parental line were identified, yielding 4.4% of mutation rate. These mutants showed modifications on any of leaf color, leaf shape, plant height, mature period, fertility, spike shape, seed-coat color and so on. Thus, these mutants would become a genetic resource being useful in deciphering the gene function and breeding for new varieties in Broomcorn millet.