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  • 李群三,陈景斌,顾和平,等.基于InDel标记的国内绿豆品种遗传多样性分析及指纹图谱构建[J].植物遗传资源学报,2019,20(1):122-128.    [点击复制]
  • LI Qun-san,CHEN Jing-bin,GU He-ping,et al.Genetic Diversity and Fingerprint Analysis of Mungbean Varieties from China Based on InDel Markers[J].植物遗传资源学报,2019,20(1):122-128.   [点击复制]
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基于InDel标记的国内绿豆品种遗传多样性分析及指纹图谱构建
李群三1,2, 陈景斌2, 顾和平2, 袁星星2, 陈新2, 崔瑾1
0
(1南京农业大学生命科学学院,南京 210095;2江苏省农业科学院经济作物研究所,南京 210014)
摘要:
绿豆是一种具有强抗逆性、适应性广的作物。本研究选取19对多态性丰富、条带稳定的引物用于全部参试样品的遗传多样性分析及指纹图谱构建。19对InDel引物有效等位基因数变幅介于1.100~1.996之间,平均为1.750;期望杂合度(He)变幅介0.0918~0.5049之间,平均为0.421;Nei’s基因多样性指数变幅介于0.0907~0.4989之间,平均为0.416。多态性信息含量PIC值变幅介于0.0866~0.3744之间,0.25<PIC<0.50为中度多态性位点,本研究所采用的InDel标记平均PIC含量为0.325。利用UPGMA构建42份主栽绿豆种质资源的聚类树状图,在遗传相似系数为0.55的阈值处可将42个绿豆品种(系)分为2大类群,遗传分析说明主栽绿豆种质资源基本已脱离地域的影响。利用19对InDel引物扩增结果,构建了42个绿豆主栽品种的DNA指纹图谱,可以将42个绿豆品种区分并准确鉴定。本研究中的InDel标记带型易读简单,适宜用于构建DNA指纹图谱和进行绿豆品种真伪鉴定,为绿豆名品种保护、原产地溯源管理等提供科学依据。
关键词:  绿豆  InDel标记  指纹图谱  遗传多样性分析
DOI:10.13430/j.cnki.jpgr.20180524002
投稿时间:2018-05-24修订日期:2018-06-05
基金项目:国家自然科学基金青年科学基金项目(31501370);国家食用豆产业技术体系(CARS-08-G15);江苏特粮特经产业技术体系(SXGC【2017】260);国家重点研发计划项目(2016YFE0203800)
Genetic Diversity and Fingerprint Analysis of Mungbean Varieties from China Based on InDel Markers
LI Qun-san1,2, CHEN Jing-bin2, GU He-ping2, YUAN Xing-xing2, CHEN Xin2, CUI Jin1
(1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095;2Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014)
Abstract:
Mungbean is a kind of crop with strong resistance, and wide adaptability. The genetic diversity analysis and fingerprint analysis of all samples were detected using 19 polymorphic and stable core markers. The effective alleles of the InDel markers were between 1.100 and 1.996, with an average of 1.750. The expected heterozygosity (He) was between 0.0918~0.5049, with an average of 0.421. The variation of Nei"s gene diversity index was between 0.0907~0.4989, with an average of 0.416. The value of the polymorphic information content (PIC) was between 0.0866~0.3744, and 0.25 < 0.50 was the medium polymorphic. The average PIC content of InDel markers used in this experiment was 0.325. Using UPGMA to construct the clustering tree of 42 varieties of mungbean germplasm resources and the result showed that, 42 mungbean varieties (lines) could be divided into 2 groups while the genetic similarity coefficient is 0.55. Genetic analysis showed that the germplasm resources of the domestic mungbean have been isolated from the effect of regional isolation. By using 19 InDel markers, the DNA fingerprint of 42 cultivars of mungbean was constructed and 42 varieties of mungbean could be distinguished and accurately identified. The bands of InDel markers are easy to read in this study and they are suitable for DNA fingerprinting. Our results provide scientific basis for the identification of varieties, the protection of varieties of mungbean and provenance management.
Key words:  Mungbean  InDel marker  fingerprint  Identification of varieties  

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