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  • 魏麦玲,裴丽丽,徐兆师.大豆MYB转录因子基因GmMYB174的克隆及分子特性分析[J].植物遗传资源学报,2015,16(1):94-99.    [点击复制]
  • Xu Zhao-Shi.Isolation and molecular characteristics analysis of soybean transcription factor gene MYB174[J].植物遗传资源学报,2015,16(1):94-99.   [点击复制]
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大豆MYB转录因子基因GmMYB174的克隆及分子特性分析
魏麦玲,裴丽丽,徐兆师
0
(中国农业科学院作物科学研究所)
摘要:
本研究克隆得到大豆转录因子MYB基因GmMYB174,该基因包含1086 bp,编码361个氨基酸,属于MYB-related家族。利用生物信息学分析表明GmMYB174与大豆、番茄、苜蓿、花生等物种同源性较高,序列分析表明它们都包含2个保守的Trp(W)位点和一个保守基序SHAQKFF。亚细胞定位结果显示GmMYB174定位于细胞核中;组织表达量显示GmMYB174在多个组织均有表达,其中在上胚轴中表达量最高。启动子元件分析表明GmMYB174包含ABRE、MYB、 MYC、LTRE、GT-1等逆境胁迫应答元件。实时荧光定量PCR分析干旱、盐、BR、ABA处理下的表达模式,结果表明GmMYB174在干旱、盐、BR、ABA处理下均有响应。因此,GmMYB174可能参与多种胁迫应答途径。
关键词:  大豆  MYB转录因子  非生物胁迫  响应机制  亚细胞定位
DOI:
投稿时间:2014-06-21修订日期:2014-08-25
基金项目:
Isolation and molecular characteristics analysis of soybean transcription factor gene MYB174
Abstract:
The full sequence of GmMYB174 was isolated from soybean, including 1086 bp and encoding 361 amino acids. GmMYB174 belonged to MYB-related family. Bioinformatics analysis indicate GmMYB174 share a high similar with tomato, alfalfa and peanut, containing 2 conserved locus and a motif of SHAQKFF. GmMYB174 located in nucleus. The expression predication of tissue show that GmMYB174 expressed in many tissues and peaked in epicotyl. Promoter analysis indicate that the region of GmMYB174 promoter contain ABRE, MYB, MYC, LTRE and GT-1. Q-RT-PCR shows that GmMYB174 involved in various abiotic stresses. GmMYB174 maybe participated in multi-environmental stress responses.
Key words:  Soybean  MYB transcription factor  abiotic stress  responsive mechanism  subcellular localization

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