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  • 陈峰,张正球,李华东,等.粳稻支链淀粉合成相关基因的遗传组成及其对品质的影响[J].植物遗传资源学报,2013,14(5):849-856.    [点击复制]
  • chenfeng,zhang zheng qiu,li hua dong,et al.Genetic Composition of Amylopectin and its Influence on Rice Qualities in Japonica varieties[J].植物遗传资源学报,2013,14(5):849-856.   [点击复制]
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粳稻支链淀粉合成相关基因的遗传组成及其对品质的影响
陈峰1, 张正球2, 李华东1, 徐建第, 高洁, 周起先1, 朱文银
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(1.山东省水稻研究所;2.连云港农业局)
摘要:
摘要:淀粉的组成和结构是决定稻米品质的主要因素。利用17个淀粉合成相关基因位点的分子标记鉴定67个粳稻品种的淀粉合成相关基因的籼粳来源,检测表明67个品种均带粳型Wx基因,GBSSⅡ、SSⅡ-2和SSⅣ-1位点没有发生重组,其他13个支链淀粉合成相关基因位点有籼型等位基因的渗入,渗入频率在1.9%-46.27%之间,其中SSⅠ籼型等位基因渗入到粳稻品种中最高;逐步回归分析表明有7个基因对10个淀粉理化指标有效应,其中Isa对最高粘度、崩解值、回复值、消减值和峰值时间有效应,其它基因只对1-4个性状有明显的遗传效应。说明在支链淀粉合成相关基因座上籼粳等位基因已经发生了明显的分化,具有不同的遗传功能,为水稻优质品种培育提供了参考依据。
关键词:  水稻  淀粉合成相关基因  淀粉理化特性,淀粉粘滞性特征
DOI:
投稿时间:2012-12-03修订日期:2013-03-18
基金项目:山东省自然科学基金(ZR2009DQ013);山东省农业良种工程项目
Genetic Composition of Amylopectin and its Influence on Rice Qualities in Japonica varieties
Abstract:
Abstract:The composition and structure of rice grain starch are two major factors which influence the grain quality. In this study, we used 17 molecular markers based on sequence diversities between two rice subspecies, indica and japonica, to investigate the genotypes of 67 japonica cultivars. The results showed: all 67 japonica varieties carried the japonica allele Wxj. , and among 16 locus of amylopectin synethesis-related genes(ASRG),13 locus had introgression of indica while the other 3 locus, GBSSⅡ, SSⅡ-2 and SSⅣ-1 , had no recombination. the frequency of indica allele introgression was from 1.9% to 46.27%, and SSⅠgene had the highest introgression frequency. Stepwise regression analysis demonstrated that 7 genes had effects on the 10 physico-chemical properties, including amylose content(AC), gel consistency(GC), starch RVA characteristics(RVA).And Isa gene had effects on peak viscosity( PKV), break down value(BDV), consistency value(CSV), setback value(SBV) and peak time(PeT),while the other 6 genes only had obvious genetic effects on 1-4 characteristics. The results indicated that the functional differentiation had been occured on the ASRG locus. These results provided useful information for rice quality improvement.
Key words:  Rice  Starch-synthesizing genes, starch physico-chemical properties, Starch RVA characteristic

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