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  • 田保华,梁毅,陈丽,等.洋葱大葱种间杂种鉴定方法建立与应用[J].植物遗传资源学报,2011,12(4):657-661.    [点击复制]
  • tianbaohua,et al.Establish and Applications of Identification Interspecific Hybrids Between Welsh Onion and Onion[J].植物遗传资源学报,2011,12(4):657-661.   [点击复制]
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洋葱大葱种间杂种鉴定方法建立与应用
田保华1, 梁毅2, 陈丽3, 王永勤2, 裴雁曦1
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(1.山西大学生命科学学院;2.北京市农林科学院蔬菜研究中心;3.华中农业大学园艺林学学院)
摘要:
以洋葱、大葱及其杂种F1为材料,利用PCR-RFLP技术对其核糖体DNA内部转录间隔区(ITS)和细胞质线粒体DNA的srRNA基因V7进行分析,试图建立洋葱大葱种间杂种的鉴定方法。结果显示:大葱ITS序列没有DdeⅠ酶切位点;而洋葱被DdeⅠ酶切成两个大小不同的片段;F1杂种具有两亲本的ITS序列酶切模式。细胞质线粒体srRNA基因的V7区域的扩增表明F1杂种和亲本有大小一致的单一片段,经RsaI酶切后,大葱有约0.3 kb条带,比洋葱的稍小,两亲本都含有另外一条小条带(约0.1 kb)。对25份杂种材料进行鉴定,22份的母本为大葱,3份为假杂种。本研究在分子水平上建立了洋葱大葱种间杂种的鉴定方法,快速、准确的辨别杂种一代的真假性。
关键词:  洋葱  大葱  种间杂种  nrDNA ITS序列  mtDNA
DOI:
投稿时间:2010-08-27修订日期:2011-04-21
基金项目:
Establish and Applications of Identification Interspecific Hybrids Between Welsh Onion and Onion
Abstract:
Abstract: Onion, Welsh Onion, and their F1 progenies were applified to establish a method for interspecific hybrids identification by using PCR-RFLP. The results showed that rDNA internal transcription spacers (ITS) of onion could be digested into two restriction fragments with endonuclease Dde I ,while no Dde I restriction site was found in Welsh onion. Welsh onion hold a restriction fragment of approximately 0.3 kb after the PCR products of V7 region of mitochondria srRNA was digested with Rsa I , which was slightly shorter than that of onion. Of the 25 F1 hybrids analyzed in this paper, 22 were true F1, while the other three ones were false hybrids. The present research established a easy and accurate method for interspecific hybrids identification between onion and Welsh onion.
Key words:  Onion  Welsh Onion  Interspecific hybrid  nrDNA ITS  mtDNA

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