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  • 忻如颖,周传珠,陈健美,等.油菜和播娘蒿体细胞杂交创造油菜高油双低新种质[J].植物遗传资源学报,2010,11(1):89-93.    [点击复制]
  • ,et al.New Canola Germplasm of High Oil Content Created by Somatic Hybrids between Brassica napus and Descurainia sophia[J].植物遗传资源学报,2010,11(1):89-93.   [点击复制]
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油菜和播娘蒿体细胞杂交创造油菜高油双低新种质
忻如颖1, 周传珠2, 陈健美1, 姜淑慧3, 张丽君4, 管荣展5
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(1.南京农业大学作物遗传与种质创新国家重点实验室,南京210095;2.江苏省东台市农业科学研究所,东台224215;3.南京农业大学作物遗传与种质创新国家重点实验室,南京210096;4.南京农业大学作物遗传与种质创新国家重点实验室,南京210097;5.南京农业大学作物遗传与种质创新国家重点实验室,南京210098)
摘要:
在甘蓝型双低油菜品种与播娘蒿原生质体融合杂种的自交后代群体中,实施定向选择和品质鉴定,在F3收获的247株中筛选获得9份含油量大于45%、芥酸含量小于0.5%、硫苷含量小于30 μmol/g的高油双低甘蓝型油菜新种质,其中有2份是黄籽高油双低材料,这些材料的F4仍保持高含油量双低性状。在F4群体收获的905株中新选出了15份高含油量双低油菜新种质,其中有5份是黄籽高油双低材料。因此,通过播娘蒿与油菜原生质体融合及其后代选择可以获得高含油量优质油菜新种质。
关键词:  双低油菜  高含油量  选育  播娘蒿  原生质体融合
DOI:
基金项目:江苏省科技支撑计划(BE2008369)
New Canola Germplasm of High Oil Content Created by Somatic Hybrids between Brassica napus and Descurainia sophia
Abstract:
Fertile progenies of somatic hybrids between double low rapeseed and Descurainia sophia were selfed in three consecutive generations,resulting in F2,F3 and F4 populations.By individual selection and quality identification ,nine elite plants with oil content more than 45%,erucic acid content less than 0.5%,glucosinolate content less than 30 μmol/g were obtained from 247 F3 individuals,two of which were yellow seeded.All of these plants remained the high oil and double-low properties in F4 population.15 new elite individuals with high oil content and double low contents were obtained from 905 F4 plants,and five of which were yellow seeded.It was concluded that through somatic hybridization and progeny selection,new germplasm with desired traits including yellow seeded,high oil percentage,double low quality,could be obtained.
Key words:  Double low rapeseed  High oil content  Selection  Descurainia sophia  Protoplast fusion

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