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  • 王凤敏,粟建光,龚友才,等.不同红麻种子耐老化性差异及热稳定蛋白的研究[J].植物遗传资源学报,2010,11(1):5-9.    [点击复制]
  • ,et al.Difference of Aging Tolerance and Heat Stable Proteins in Seeds of Difference Kenaf Cultivars[J].植物遗传资源学报,2010,11(1):5-9.   [点击复制]
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不同红麻种子耐老化性差异及热稳定蛋白的研究
王凤敏, 粟建光, 龚友才, 戴志刚, 陈基权, 郑海燕, 李燕
0
(中国农业科学院麻类研究所,长沙410205)
摘要:
利用10份红麻品种为材料,研究红麻种子在人工老化过程中耐老化差异及其与热稳定蛋白的关系。结果表明:(1)随着老化处理程度加深,10份红麻品种种子的发芽率、发芽指数及活力指数均逐渐下降,但品种之间有显著差异;(2)热稳定蛋白含量随着老化处理的加深呈现上升的趋势,通过SDS-PAGE电泳显示,辽55在140h老化处理中出现一条差异带,分子量为62.8kD,其他9个品种各个处理间未出现差异带。10份红麻品种在老化过程中,辽55最耐老化,在辽55中发现的热稳定蛋白可能与红麻品种的耐老化性及种子活力的丧失有关。
关键词:  红麻  种子  耐老化  热稳定蛋白
DOI:
基金项目:国家作物种质资源保护项目(NB08-2130135-40)
Difference of Aging Tolerance and Heat Stable Proteins in Seeds of Difference Kenaf Cultivars
Abstract:
The difference of aging tolerance and the heat stable proteins were among 10 kenaf(Hibiscus cannabinus L.)cultivars,though aging treatment of kenaf.The results indicated that,(1)Seed gemmation and vigor index of 10 kenaf varieties reduced with the extent time in the process of aging,while there were notable difference in 10 kenaf varieties;(2)The content of heat stable protein gradually increased with aging treatment deepening,SDS-PAGE eletrophoresis showed that a 62.8kD heat stable protein appeared only in liao 55 in 140h aging treatment,there were no differences in others.Liao 55 was the most anti aging variety during aging treatment in 10 kenaf varieties,these results suggested that the special heat stable proteins might be related to aging tolerance.
Key words:  Kenaf  Seed  Aging tolerance  Heat stable protein

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