This experiment was carried out in examination base of Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. The effects of different N, P, K, S fertilizers on high gluten wheat yield and quality were researched. The result showed that the grain yield, kilo-kernel weight and liter weight were the highest when N fertilizer treatment was 120kg/hm^2 at the high yield condition. But they had depressed trend when excess N fertilizer applied. The grain rigidity was increased and protein content, wet gluten content, development time and stable time all have increased trend, and bread volume was remarkable increased with the amount of N-fertilizer added. While protein content, wet gluten content and stable time showed increasing trend with the amount of K-fertilizer added. There was no obvious effect of P and S fertilizer treatments on grain yield and quality in the experiment.