烟草黄绿叶突变体的遗传分析与基因定位
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1中国农业科学院烟草研究所,山东青岛266100;,1中国农业科学院烟草研究所,山东青岛266100;,2中国烟草总公司郑州烟草研究院,郑州450001;,2中国烟草总公司郑州烟草研究院,郑州450001;,2中国烟草总公司郑州烟草研究院,郑州450001;,3湖南中烟工业有限责任公司,长沙410007,3湖南中烟工业有限责任公司,长沙410007,1中国农业科学院烟草研究所,山东青岛266100;,1中国农业科学院烟草研究所,山东青岛266100;

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中国烟草总公司烟草基因组计划重大专项(110201601028(JY-02));中国农业科学院科技创新工程(ASTIP-TRIC01);中国烟草总公司烟草科技重点项目(110201502012)


Genetic Analysis and Mapping of a Yellow-green Leaf Mutant of Tobacco (Nicotiana tabacum L.)
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1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;

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    摘要:

    以G117为父本,RG13为母本,杂交获得F<sub>1</sub>群体。系谱法常规选择过程中,在F<sub>3</sub>代株系内发现黄绿自然隐性突变株。该突变体的叶色在旺长期前呈现正常绿色,进入旺长期后,叶色逐渐发黄,叶脉呈乳白色,与正常烟株差别明显。遗传分析表明该突变体性状受一对隐性基因控制。从来源于一个连续自交单株的分离群体中分别选取10份隐性纯合烟株和10份显性烟株,利用430K烟草高密度SNP芯片进行基因型分析,快速确定了与目标性状相关联的标记。进而利用该分离群体验证相关分子标记,将该基因定位在烟草第5号染色体M7和M18之间,并与M7标记共分离。相关研究为进一步克隆该基因奠定了基础,同时也为烟草其他重要性状的定位提供了一种有效、快速的方法。

    Abstract:

    In this study, F<sub>1</sub> population was constructed by a cross derived from tobacco cultivars G117 and RG13. A natural mutant individual was found in F<sub>3</sub> lines during breeding selection. The mutant exhibits a yellow-green leaf phenotype, which is controlled by one recessive gene by classical genetic analysis. Significant markers related with aimed trait were detected by jointly 430 K SNP chip with two extreme selective populations. Subsequently, the entire segregation population derived from one F<sub>7</sub> plant was genotyped using PCR method at these significant markers loci. Finally, the recessive gene controlled a yellow-green leaf phenotype in tobacco was mapped to chromosome 5 and co-segregated with the SNP marker M7. These results could enhance our understanding of the inheritance of yellow-green leaf mutant, and provide a new avenue for mapping the mutant loci or the QTL in tobacco by combining SNP chip and extreme selection populations.

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孙明铭,蒋彩虹,罗朝鹏,等.烟草黄绿叶突变体的遗传分析与基因定位[J].植物遗传资源学报,2018,19(5):942-950.

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  • 收稿日期:2018-01-31
  • 最后修改日期:2018-06-23
  • 录用日期:2018-05-21
  • 在线发布日期: 2018-07-05
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