基于SSR标记的132份甘薯种质指纹图谱的构建及遗传多样性分析
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1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;2山东大学生命科学院,济南 250100,1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;,1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;,1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;,1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;,1山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,济南 250100;

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山东省良种工程项目(2016LZGC005),国家甘薯产业技术体系(CARS-10-B07),山东省重点研发计划项目(2018GNC111016),山东省现代农业产业技术体系薯类产业创新团队遗传育种岗位(SDAIT-10-022-04)


Establishment of DNA Fingerprinting for 132 Sweetpotato Cultivars by SSR Markers
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1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;2 School of Life Science, Shandong University, Jinan 250100,1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;,1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;,1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;,1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;,1Crop Research Institute, Shandong Academy of Agricultural Sciences/Scientific Observiing and Experimental Station ofTuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture,Jinan 250100;

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    摘要:

    利用SSR分子标记技术,构建132份甘薯种质的DNA指纹图谱,并进行遗传多样性分析,旨在为甘薯种质资源亲缘关系鉴定、分类提供理论依据。利用筛选的核心引物进行PCR扩增,通过聚丙烯酰胺凝胶电泳检测显示,19对引物共扩增出232个条带,其中多态性条带165条,多态性比率为71.1%,平均每对引物扩增出8.68个条带,多态性信息含量变化范围在0.6706~0.9331,平均0.8158;其中引物SSR9和引物C33可将132份种质完全区分开,并构建供试材料的DNA指纹图谱,供试材料遗传距离在0.0363~0.5939之间,平均为0.4087,表明种质资源间遗传多样性丰富。基于SSR标记对供试材料进行聚类分析,将供试材料分为4亚类,第1类由济薯25与3份日本引进品种组成,第2类由济徐薯23、苏丹、济薯09281组成,第3类由37份S07材料和18份甘薯种质组成,第4类由剩余的70份种质组成,为甘薯分子辅助育种中亲本的选择提供理论依据。

    Abstract:

    To provide a theoretical basis for the identification and classification of 132 sweetpotato germplasm accessions, the DNA fingerprints and the genetic diversity were analyzed using SSR markers. As a result, there were 232 fragments were obtained by PCR amplification using 19 pairs of primers , including 165 were polymorphic bands with a polymorphism ratio of 71.1% and an average of 8.68 bands and with a polymorphism ratio of 71.1%. The polymorphic information content values (PIC) ranged from 0.6705 to 0.9331, with a mean of 0.8158. Notably, these 132 varieties could be assigned completely by using the primer pairs SSR9 and C33 and , thus both markers could be valuable for constructing DNA fingerprints. The genetic distance of 132 sweetpotato germplasms ranged from 0.0363 to 0.5939 with a mean of 0.4087, indicating a high level of genetic diversity. The cluster analysis revealed that the tested materials were divided into four categories. For example, the first category consisted of Jinshu 25 and three Japan introduced varieties, the second category consisted of JiXushu 23, Sudan, and Jinshu09281, the third category consisted of 37 S07 genotypes and 18 sweetpotato accessions, and the fourth category consisted of the remaining 70 accessions. Thus, this work deciphered the genetic diversity of the tested materials, which might provide a theoretical basis for selecting the parental lines used for molecular assisted breeding of sweetpotato.

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聂立圆,李爱贤,秦桢,等.基于SSR标记的132份甘薯种质指纹图谱的构建及遗传多样性分析[J].植物遗传资源学报,2018,19(5):904-911.

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  • 收稿日期:2018-01-23
  • 最后修改日期:2018-05-12
  • 录用日期:2018-03-30
  • 在线发布日期: 2018-09-14
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