模拟干旱胁迫下马铃薯StNCED1表达量及与ABA含量的相关性分析
作者:
作者单位:

宁夏农林科学院农业生物技术研究中心,黑龙江省农业科学院克山分院,宁夏农林科学院农业生物技术研究中心,宁夏农林科学院农业生物技术研究中心,宁夏农林科学院固原分院,宁夏农林科学院农业生物技术研究中心

作者简介:

通讯作者:

中图分类号:

基金项目:


Correlation analysis of StNCED1 expression level and ABA content of potato under simulated drought stress
Author:
Affiliation:

Agricultural Bio-Technology Centre,Ningxia Academy of Agriculture and Forestry Science,Keshan Branch of Heilongjiang Academy of Agricultural Science,Agricultural Bio-Technology Centre,Ningxia Academy of Agriculture and Forestry Science,Agricultural Bio-Technology Centre,Ningxia Academy of Agriculture and Forestry Science,Guyuan Institute of Agricultural Sciences,Ningxia Academy of Agriculture and Forestry Science,Agricultural Bio-Technology Centre,Ningxia Academy of Agriculture and Forestry Science

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    干旱缺水是限制马铃薯产量和品质的关键因素之一;ABA是干旱胁迫应答基因调控网络中的重要组成;9-顺式-环氧类胡萝卜素双加氧酶(NCED)是植物ABA生物合成途径的关键限速酶,该基因的表达模式直接影响ABA代谢。但干旱胁迫下有关马铃薯NCED基因表达与激素、表型间的相关分析的研究尚少。本研究克隆了马铃薯NCED1基因的全长cDNA序列,检测了4个马铃薯种质材料在不同模拟干旱(PEG‐6000)胁迫强度处理下NCED1基因表达量、ABA 含量及根系长度间的相关性。结果表明,StNCED1全长2181 bp,包含一个1800 bp的完整开放读码框,编码599个氨基酸。5%PEG‐6000和15% PEG‐6000模拟干旱胁迫4周下,4个品种均表现出随胁迫强度的加重植株生长缓慢、矮小,根系长度显著降低。干旱敏感型材料“早大白”中根系长度变化最大,ABA含量显著高于其他3个种质材料。CIP478.9、star和米拉3份种质材料中StNCED1表达量与对照植株有显著差异,且随胁迫强度增加而增大;“早大白”中StNCED1表达量表现出先降低后升高趋势。模拟干旱胁迫下,ABA含量与StNCED1表达量之间呈现出正相关关系(R>0.7)。研究结果为解析马铃薯响应干旱胁迫的调节机制及其在抗旱种质资源筛选中的应用提供了基础数据。

    Abstract:

    Drought is one of the most critical factor limiting the potato yield and quality. Abscisic acid (ABA) is the essential part of drought responsing regulatory networks and 9-cis-epoxycarotenoiddioxygenase (NCED) is the keyrate-limiting enzyme ofABA biosynthesis in higher plants.It directly influences the expression pattern of ABA metabolism. However, there are few reports on relationship among NCED expression pattern, ABA content and phenotypic changes on potato. In this research, A NCED gene (StNCED1)has been cloned from potato and NCED expression levels, ABA content and root length changes was evaluated in 4 varieties under simulated drought stress by different concentration of PEG‐6000. The results showed that the full length of StNCED1 was 2181 bp, which contains a 1803 bpopen reading frame (ORF) encoding a protein of 600 amino acid residues.Analysis showed that all the 4 varieties seedling growth was significantly inhibited, and especially the root length decreased sharply with the stress concentration increased from 5% upto 15%. Drought sensitive material“zaodabai” showed the most obvious changes in root length among the 4 tested materials and the ABA content is significantly higher than the other three materials. The expression level of StNCED1 in drought stressed plant was significantly higher than the control group of CIP478.9, star and mila, respectively. The expression levels of were increased with increasing concentration of PEG‐6000 in these three materials. On the opposite, StNCED1 expression and ABA content showed a decrease at first and then an increase trend in “zaodabai”. A positive correlation (R>0.7) was proved between ABA concentration change and StNCED1 expression level in all the 4 tested materials under drought stress. Our results will provide fundermental data for further revealing the regulation mechanism ofStNCED1 responsing to drought stress and will be helpful in screening drought resistance germplasm resources.

    参考文献
    相似文献
    引证文献
引用本文

巩檑,宋继玲,甘晓燕,等.模拟干旱胁迫下马铃薯StNCED1表达量及与ABA含量的相关性分析[J].植物遗传资源学报,2018,19(3):561-567.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-10-26
  • 最后修改日期:2018-03-15
  • 录用日期:2017-12-28
  • 在线发布日期: 2018-05-08
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司