引用本文
  • 雷启义,熊勇,周江菊,张文华,孙军,龙春林.黔东南香禾糯AFLP遗传多样性分析与评价[J].植物遗传资源学报,2017,18(6):1023-1031.    [点击复制]
  • Lei Qiyi,周江菊,张文华,孙军,Long Chunlin.Genetic diversity analysis and evaluation of Kam aromatic glutinous rice in Qiandongnan Prefecture based on AFLP molecular markers[J].植物遗传资源学报,2017,18(6):1023-1031.   [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 213次   下载 436 本文二维码信息
码上扫一扫!
黔东南香禾糯AFLP遗传多样性分析与评价
雷启义1,2, 熊勇1,2, 周江菊3, 张文华3, 孙军3, 龙春林1,4
0
(1.中央民族大学生命与环境科学学院;2.凯里学院;3.凯里学院环境与生命科学学院;4.中国科学院昆明植物研究所)
摘要:
香禾糯是我国侗族地区最重要的农作物之一,是侗族数百年来的传统主食传统农业生态系统的重要组成部分。当前,贵州黔东南侗族地区仍然保持着丰富的香禾糯种质资源,从分子水平探讨该地区香禾糯遗传多样性及其形成背景,为香禾糯种质资源的保护与利用提供参考。通过民族植物学调查与现代分子生物学实验相结合的方法,对黔东南侗族地区传统分类的香禾糯农家品种遗传多样性行评价。利用AFLP分子标记方法,对95个香禾糯农家品种的基因组DNA进行酶切、连接、预扩增、选择性扩增,3730XL测序仪进行毛细管电泳,使用PopGene1.32软件计算每对引物的多态性条带数、多态性比率等指标,用NTSYS pc-V. 2.1 计算种质间遗传相似系数,对香禾糯种质资源进行聚类分析。选用的8对组合引物进行扩增,检测到群体总位点数为707个,多态性位点为619个,多态性位点比例为87.55%,Shannon-Weiner 多样性指数为0.3738;8个侗寨的香禾糯遗传相似性系数为0.9484-0.9860,其中黄岗和占里侗寨的最高,达0.9860;侗寨间群体的遗传距离为0.0141-0.0529,坑洞和黄岗的遗传距离最大,遗传距离与地理位置不相关。95个品种的遗传相似系数为0.7121-0.9958,当遗传相似系数为0.7546时,可划分为 2 大类( A和B),外加1个特殊品种,其中A类群有88个品种,B类群有6个品种,来自相同和相邻侗寨或者形态性状相近的品种并没有完全聚在一起。结果表明黔东南地区香禾糯农家品种遗传多样性总体水平较高,特别是传统耕作文化保护较好的侗寨内,其香禾糯遗传多样性较为丰富,遗传背景差异较大,民间传统分类对于了解地方水稻品种的多样性具有很重要的参考价值。香禾糯种质资源的多样性形成,在很大程度上与黔东南地区复杂多变的自然环境有关,还与该地区少数民族的传统耕作制度和民族传统文化延续密切相关。
关键词:  香禾糯  遗传多样性  AFLP  种质资源  传统文化  民族植物学
DOI:10.13430/j.cnki.jpgr.2017.06.004
投稿时间:2017-05-26修订日期:2017-08-30
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
Genetic diversity analysis and evaluation of Kam aromatic glutinous rice in Qiandongnan Prefecture based on AFLP molecular markers
Abstract:
Crop landraces are an important component of crop genetic resources, which is of great significance to food security and people’s livelihood, thus becoming the main target of germplasm resource conservation and rural development. The Kam aromatic glutinous rice is one of the most important crops in Dong ethnic areas of China. It has become an important component of Dong ethnic traditional staple food crop for hundreds of years. At present, there are abundant germplasm resources of Kam aromatic glutinous rice in Qiandongnan Miao and Dong Autonomous Prefecture, Guizhou Province, China. Aimed at providing data for the conservation and utilization of Kam aromatic glutinous rice germplasm resources, the genetic diversity and similarity of Kam aromatic glutinous rice were explored at the molecular level in the area. In the study, an approach combining ethnobotany investigation and molecular biological experiment was used to evaluate the genetic diversity of the Kam aromatic glutinous rice landraces in Dong ethnic area of Qiandongnan Prefecture. The genomic DNA was extracted from young leaves of 95 Kam aromatic glutinous rice landraces collected from Dong villages following the CTAB method, and then AFLP (amplified fragment length polymorphism) molecular markers was adopted for enzyme digestion analysis, co??????nnection and pre-amplification and selective amplification, and 3730 XL sequencing facility was used for capillary electrophoresis. The polymorphic loci, percentage of polymorphic loci, genetic distance and genetic similarity were analyzed through PopGene1.32 and NTSYS pc-V. 2.1 software. The Kam aromatic glutinous rice landraces were clustered with UPGMA (unweighted pair group method analysis) method. A total of 707 clear bands were obtained, 619 of them were polymorphic bands and the percentage of polymorphic was 87.55%. Shannon’s information index was 0.3738. The genetic similarity coefficient of 8 populations of Kam aromatic glutinous rice is 0.9484-0.9860. The genetic distance was 0.0141-0.0529. The genetic similarity coefficient of Zhanli and Huanggang was the highest, while the genetic distance of Kengdong and Huanggang was the forest. There was no correlation between genetic distance and geographic distance. The genetic similarity coefficient of the 95 accessions was 0.7121-9958. It could be clustered into 2 big clusters (A and B) when the genetic similarity coefficient was 0.7546, with the addition of a special group of landraces. Branch A included a group of 88 landraces, and Branch B had a total of 6 landraces. The varieties were collected from the same and adjacent villages or the varieties with similar morphological traits that were not clustered completely together, there was no correlation between the genetic distance and the geographical location. The results show that there was higher genetic diversity in the landraces of Qiandongnan Prefecture, especially in the Dong villages where the genetic differentiation was high, and the genetic background was very complex in the Kam aromatic glutinous rice landraces. In addition, ethnic traditional classification has important reference value for understanding the diversity of rice landrace varieties. The formation of diversity was related to complex environment, ethnic traditional farming system and culture in the prefecture. The AFLP molecular system establishment and the reveals of diversity background of Kam aromatic glutinous rice provided a basis for the follow-up correlative study of the germplasm resources, which is conducive to further excavate and utilize the especial germplasm resources and provides an important basis for formulating relevant protection strategies.
Key words:  Kam aromatic glutinous rice  genetic diversity  AFLP  germplasm resources  traditional culture  ethnobotany

用微信扫一扫

用微信扫一扫