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  • 陈红霖,胡亮亮,王丽侠,王素华,程须珍.绿豆bHLH转录因子家族的鉴定与生物信息学分析[J].植物遗传资源学报,2017,18(6):1159-1167.    [点击复制]
  • CHEN Hong-Lin,HU Liang-liang,WANG Li-Xia,WANG Su-Hua,CHENG Xu-Zhen.Genome-Wide Identification and Bioinformatics Analysis of bHLH Transcription Factor Family in Mung Bean (Vigna radiata L.)[J].植物遗传资源学报,2017,18(6):1159-1167.   [点击复制]
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绿豆bHLH转录因子家族的鉴定与生物信息学分析
陈红霖,胡亮亮,王丽侠,王素华,程须珍
0
(中国农业科学院作物科学研究所)
摘要:
bHLH是真核生物中重要的一类转录因子,其主要由碱性氨基酸区和螺旋-环-螺旋区组成。本文利用生物信息学的方法鉴定到122个绿豆bHLH转录因子,并对其理化性质、保守结构域、基因结构、在染色体上的分布、系统进化以及部分典型基因的组织表达差异等进行分析。结果表明,bHLH转录因子理化性质差异较大;含有2个保守结构域,分别位于N端的碱性氨基酸区和C端的螺旋-环-螺旋区,碱性氨基酸区含有His5-Glu9-Arg13保守序列,与靶基因结合有关,HLH区含有Arg23和Arg55,与形成二聚体有关,同时含有5种保守元件;bHLH基因在11条染色体上分布不均匀,5号、7号和8号染色体上分布较多,1号、4号和10号染色体上分布较少,大部分基因含有1-9个不等的内含子,在染色体上成簇状分布;122个bHLH转录因子可分为11个亚家族。多数bHLH基因在绿豆根、茎、叶、花和种子等组织中都有表达,但具有组织表达特异性,且不同基因表达量差异较大。本研究为进一步研究绿豆bHLH转录因子家族的生物学功能奠定基础。
关键词:  绿豆  bHLH  转录因子  生物信息学分析
DOI:10.13430/j.cnki.jpgr.2017.06.018
投稿时间:2017-04-01修订日期:2017-08-09
基金项目:
Genome-Wide Identification and Bioinformatics Analysis of bHLH Transcription Factor Family in Mung Bean (Vigna radiata L.)
CHEN Hong-Lin,HU Liang-liang,WANG Li-Xia,WANG Su-Hua,CHENG Xu-Zhen
(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences)
Abstract:
Basic helix-loop-helix protein (bHLH) is an important transcription factor in eukaryotes, and it is mainly composed of basic amino acid region and helix-loop-helix region. In this study, a total of 122 mung bean bHLH transcription factors were identified by bioinformatical methods, and their physicochemical properties, conserved domain, gene structure, chromosome distribution, family evolution and expression differences of some typical bHLH genes were analyzed. The results show their physicochemical properties were different. The bHLH contained two conserved domains, which were located in the alkaline amino acid region of N and the helical ring and spiral region of C, respectively. There were five conserved motifs, and the basic amino acid region contained the conserved sequence: His5-Glu9-Arg13, and they were related to the binding with the target gene, while HLH region contained two conserved residues, Arg23 and Arg55, which were related to the formation of dimer. The bHLH genes were uneven distribution on the 11 chromosomes in mung bean. The number of genes on chromosome 5, 7 and 8 were larger, and the number of genes on chromosome 1, 4 and 10 were smaller. The number of introns ranging from 1 to 9, and the genes were clustered on some chromosomes. The phylogenetic analysis showed that 122 bHLH transcription factors were divided into 11 subgroups. Our results showed that the expression of bHLH is diverse and exhibit distinct patterns in different tissues of mung bean. This study lays a foundation for further study on the complex biological functions of bHLH transcription factor family in mung bean.
Key words:  Mung bean  Basic helix-loop-helix  Transcription factor  Bioinformatics analysis

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