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  • 吴丽艳,郭志祥,曾莉,鲍锐,黎志彬,龚亚菊.云南野生茄资源黄萎病苗期人工接种抗性鉴定分析[J].植物遗传资源学报,2017,18(6):1046-1054.    [点击复制]
  • wuliyan,郭志祥,曾莉,鲍锐,黎志彬,龚亚菊.Resistance identification of Yunnan wild eggplant resources to Verticillium wilt[J].植物遗传资源学报,2017,18(6):1046-1054.   [点击复制]
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云南野生茄资源黄萎病苗期人工接种抗性鉴定分析
吴丽艳,郭志祥,曾莉,鲍锐,黎志彬,龚亚菊
0
(云南省农业科学院园艺作物研究所;云南省农业科学院农业环境资源研究所)
摘要:
黄萎病是目前茄子生产中的主要病害之一,广泛收集、鉴定、筛选抗性资源,尤其是从野生近缘种发掘抗性基因并培育抗病品种,是解决茄子黄萎病危害的优选途径,云南省拥有丰富的野生茄子资源但尚未有效利用。本研究针对云南省茄子主产区收集到的3种黄萎病菌株,通过形态学鉴定和真菌18S rDNA/ITS鉴定,均属于大丽轮枝菌,并通过致病力鉴定,筛选出一株强致病力菌株(QZ-S);应用菌株QZ-S,通过苗期人工接种的方法对45份云南野茄资源开展黄萎病抗性鉴定,最终筛选出2份高抗材料(蒜芥茄和喀西茄),2份抗病材料(水茄和多裂水茄),6份中抗材料(1份刺天茄和5份红茄);此外,还筛选到了1份黄萎病高感材料239-3-2。本研究筛选出的材料可应用于茄子黄萎病抗病育种,为茄子及其它作物黄萎病抗病育种提供抗源。
关键词:  云南  野茄资源  大丽轮枝菌  黄萎病  抗性鉴定
DOI:10.13430/j.cnki.jpgr.2017.06.007
投稿时间:2017-03-15修订日期:2017-06-06
基金项目:国家自然科学基金地区科学基金项目“云南野茄资源黄萎病抗性机理研究(31460512)”
Resistance identification of Yunnan wild eggplant resources to Verticillium wilt
wuliyan,郭志祥,曾莉,鲍锐,黎志彬,龚亚菊
()
Abstract:
Verticillium wilt seriously influences the yields and qualities of eggplant. Studies found that extensive collection, identification and screening of the resistant materials especially from the wild resources, was the preferred option to solve the harms of Verticillium wilt. In Yunnan province, it is rich in wild eggplant resources, but these resources haven’t been utilized effectively. In this study, 3 purified isolates from infected plants were collected from the main production area of Yunnan Province, and they were determined as Verticillium dahliae Kleb. based on morphological and molecular identifications. Afterwards, one of the strains (QZ-S) was selected by resistance evaluation and to be used in the following inoculation experiment. The results found that, among 45 Yunnan wild eggplant resources that were inoculated with strain QZ-S, 2 materials ( Solanum sisymbrifolium and S. aculeatissimum ) showed high disease resistance, 2 materials ( S. torvum and S. chrysotrichum ) showed disease resistance and 6 materials ( including 1 S. violaceum material and 5 S. integrifolium materials) showed moderate resistance, and a high susceptibility material (239-3-2) was selected for its high disease percentage and disease index. All of the materials that were selected in this study could be appropriately utilized as disease resistance breeding resources. Besides, this study could broaden the germplasm resource of eggplant, lay the foundation for shortening the breeding period and providing the resistent resources for eggplant and the other crops.
Key words:  Yunnan  Wild eggplant resources  Verticillium dahliae Kleb  Verticillium wilt  Resistance identification

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