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  • 冯志娟,徐盛春,刘娜,张古文,胡齐赞,龚亚明.菜用大豆CIPK基因对逆境胁迫及激素的响应特征[J].植物遗传资源学报,2017,18(6):1168-1178.    [点击复制]
  • Feng Zhijuan,Xu Sheng-chun,Liu Na,Zhang Gu-wen,Hu Qi-zan,Gong Yaming.Expression character of vegetable soybean CIPKs in response to abiotic stresses and hormones[J].植物遗传资源学报,2017,18(6):1168-1178.   [点击复制]
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菜用大豆CIPK基因对逆境胁迫及激素的响应特征
冯志娟,徐盛春,刘娜,张古文,胡齐赞,龚亚明
0
(浙江省农业科学院蔬菜研究所)
摘要:
CIPK(calcineurin B-like-interacting protein kinase)是一类丝氨酸/苏氨酸蛋白激酶,在植物响应逆境胁迫和激素信号转导中发挥重要作用。本文利用大豆基因组数据库,在全基因组水平鉴定到CIPK蛋白激酶52个。蛋白比对分析发现所有GmCIPK含有高度保守特征性的N端激酶区、连接区和C端调控区。系统进化树分析发现大豆GmCIPK与拟南芥、水稻CIPK分类一致,分为 4个亚家族,且每个亚家族含有三个不同物种的成员,表明GmCIPK基因的分化早于物种的分化。启动子分析表明,多数GmCIPK基因的启动子区含有逆境和激素应答元件。组织表达分析发现,GmCIPK基因呈现多样化的组织表达特性。进一步选取组织表达量相对较高的14个GmCIPK进行荧光定量PCR分析,结果表明这些菜用大豆CIPK基因在不同程度上均受高温、干旱、高盐胁迫以及ABA、ACC、SA、MeJA激素的诱导表达。采用同源比对和蛋白互作在线数据库对拟南芥及大豆同源CIPK蛋白激酶与其他蛋白的互作关系进行了预测分析。本研究为菜用大豆中CIPK基因的功能研究与利用奠定了基础。
关键词:  菜用大豆  CIPK蛋白激酶  逆境应答  激素应答  蛋白互作
DOI:10.13430/j.cnki.jpgr.2017.06.019
投稿时间:2017-03-01修订日期:2017-04-10
基金项目:国家自然科学基金项目(31601767和31572138);浙江省农业科学院青年人才培养项目(2016R23R08E06);浙江省公益项目 (2015C32050)
Expression character of vegetable soybean CIPKs in response to abiotic stresses and hormones
Feng Zhijuan,Xu Sheng-chun,Liu Na,Zhang Gu-wen,Hu Qi-zan,Gong Yaming
(Institute of Vegetables, Zhejiang Academy of Agriculture)
Abstract:
Calcineurin B-like-interacting protein kinase (CIPKs) was one kind of serine/threonine kinases, playing crucial roles in abiotic stresses and hormones signal transduction pathways. Based on soybean genome database, a total of 52 CIPK genes were identified from soybean. Multiple alignments showed that all soybean CIPK proteins had the conserved regions which were composed of an N-terminal kinase domain, a junction region and a C-terminal regulatory domain. Phylogenetic analysis of CIPKs form soybean, Arabidopsis and rice revealed that all CIPKs were classified into four subfamilies and each subfamily was composed of homologous CIPKs from these three species. The classification of CIPKs from soybean was consistent with Arabidopsis and rice, which implied that the differentiation of CIPK genes occurred before the evolution of plant species. Promoter sequences analysis suggested that most GmCIPKs had stress and hormone related cis-acting elements in their promoter regions. Tissues expression analysis revealed that each GmCIPK had different expression pattern in different tissues. Furthermore, 14 GmCIPKs with relatively higher transcript level in different tissues were selected to investigate their transcriptional response to various stimuli by real-time PCR. The results showed the comprehensive responses of the tested GmCIPKs to heat, drought, salt stressors and ABA, ACC, SA, MeJA signaling. By BLASTP analysis, 17 orthologous GmCIPKs involved in CIPK interaction networks were identified between Arabidopsis and soybean. The findings of this study will provide a foundation for the functional characterization of the GmCIPK genes in vegetable soybean.
Key words:  vegetable soybean  CIPK protein kinase  abiotic stress response  hormone response  protein interaction

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