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  • 杨好慧,邱杨,宋江萍,张晓辉,王鸿娜,王超楠,王海平,李锡香.萝卜种质资源对芜菁花叶病毒的抗性鉴定与评价[J].植物遗传资源学报,2017,18(5):810-818.    [点击复制]
  • yanghaohui,邱杨,songjiangping,张晓辉,王鸿娜,王超楠,王海平,李锡香.Identification and Evaluation for The Resistance of Radish (Raphanus sativus L.)Germplasm Resources to turnip mosaic virus[J].植物遗传资源学报,2017,18(5):810-818.   [点击复制]
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萝卜种质资源对芜菁花叶病毒的抗性鉴定与评价
杨好慧,邱杨,宋江萍,张晓辉,王鸿娜,王超楠,王海平,李锡香
0
(中国农业科学院蔬菜花卉研究所;中国农科院蔬菜所;中国农业科学院蔬菜所)
摘要:
摘要:病毒病是危害萝卜作物的主要病害之一,芜菁花叶病毒(TuMV)作为萝卜病毒病的主要毒源,给萝卜生产造成严重经济损失。由于缺乏精准鉴定,萝卜育种中缺乏稳定可靠的抗TuMV材料。本研究就国家蔬菜种质资源中期库提供的来自全国25个省份125个县市和其他3个国家的150份代表性萝卜种质资源对TuMV的抗性进行了苗期鉴定和ELISA检测,筛选出23份抗病材料,其中1份表现免疫。这些材料对萝卜抗病毒病基因定位和新品种培育具有重要意义。
关键词:  萝卜种质  芜菁花叶病毒  抗性鉴定  ELISA
DOI:10.13430/j.cnki.jpgr.2017.05.002
投稿时间:2017-02-13修订日期:2017-05-02
基金项目:十三五国家重大研发项目(2016YFD0100204-02);十二五国家科技支撑项目 (2013BAD01B04-1) ;中国农科院创新工程项目 (CAAS-ASTIP-IVFCAAS).
Identification and Evaluation for The Resistance of Radish (Raphanus sativus L.)Germplasm Resources to turnip mosaic virus
yanghaohui,邱杨,songjiangping,张晓辉,王鸿娜,王超楠,王海平,李锡香
(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences)
Abstract:
Abstract: Virus disease is one of the major disease of radish (Raphanus sativus L.). Turnip mosaic virus (TuMV) which is the most important causative virus causes great economical loss in radish. The virus-resistant materials in radish breeding are indispensable currently. In this study, the resistance to TuMV was identified among 150 accessions of representative radish germplasm, coming from 125 cities of 25 provinces in China and other 3 countries. As a result, one immune materials among 23 resistant materials were screened out by combing phenotyping and ELISA detection. These materials would be useful for resistant gene mapping and new radish variety development in future.
Key words:  Radish germplasm  TuMV  resistant phenotyping,  ELISA

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