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  • 李晓华,丁明亮,乔玲,李刘军,杨堤贻,赵佳佳,曹勇,郑军,杨三维.粗山羊草CCT基因家族进化及节律表达分析[J].植物遗传资源学报,2017,18(6):1151-1158.    [点击复制]
  • 李晓华,丁明亮,乔玲,李刘军,杨堤贻,赵佳佳,曹勇,郑军,杨三维.Evolution and rhythm expression research on CCT family genes of Aegilops tauschii[J].植物遗传资源学报,2017,18(6):1151-1158.   [点击复制]
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粗山羊草CCT基因家族进化及节律表达分析
李晓华1, 丁明亮2, 乔玲3, 李刘军1, 杨堤贻3, 赵佳佳3, 曹勇4, 郑军4,4,4,4, 杨三维3
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(1.山西大学生物工程学院;2.云南省农业科学院粮食作物研究所;3.山西省农科院小麦研究所;4.山西省农业科学院小麦研究所)
摘要:
CCT家族基因广泛参与植物花期的调控过程,粗山羊草(Aegilops tauschii)作为小麦D基因组供体,给小麦带来新的花期及适应性相关基因。研究粗山羊草CCT家族基因不仅可为小麦进化、驯化和演变规律提供参考,还有助于认识粗山羊草作为杂草的生态适应性。粗山羊草基因组中26个CCT基因进化分析后发现GroupA,C,H和G中的13个AetCCT成员出现了快速进化;GroupA中有42.1%的位点存在正选择效应,表明快速进化提高了粗山羊草的适应性。基因结构分析表明CCT结构域在AetCCT家族中保守性很高,但不同基因内含子和外显子的排布差异较大,特异motif可能是不同亚家族基因间功能差异的重要原因。AetCCT4、7、8、11、12、16、17、19和21的表达具有明显的“生物钟效应”,呈现出24h的节律性表达,且基本都处于快速进化的GroupA,C,H和I。研究结果表明,这些成员可能参与花期调控等生长发育过程,在粗山羊草的适应性形成过程中发挥了作用。
关键词:  粗山羊草  CCT基因家族  正选择效应  生物钟效应
DOI:10.13430/j.cnki.jpgr.2017.06.017
投稿时间:2017-02-12修订日期:2017-04-05
基金项目:国家自然科学青年基金(31601307); 山西省农业攻关项目(20150311001-5); 山西省自然科学基金 (2016011001)。
Evolution and rhythm expression research on CCT family genes of Aegilops tauschii
Abstract:
CCT family gene widely participates in the regulating process of flowering of plants. The research on CCT gene of Ae. tauschii may provide a reference for exploring the wheat's evolution, domestication and also help to understand the ecological adaptability of Ae. tauschii. The AetCCT were divided into ten subgroups based on phylogenetic analysis. Results of phylogenetic analysis show that 13 AetCCT members of Group A, C, H and G achieved rapid evolution. After normal selection effect of some branches, rapid evolution improves the adaptability of Ae. tauschii. The conserved motifs analysis suggest that AetCCT genes in the same Group may have similar function, the specific motif of different members among families and within family is likely to be the important reason for the different functional differences among different subfamily genes. The expression of AetCCT4, 7, 8, 11, 12, 16, 17, 19 and 21 has obvious biological clock effect, with the 24h rhythm expression, so it is speculated that these members are involved in regulating the growth and development such as flowering.
Key words:  Aegilops tauschii  CCT family genes  Positive selection  Biological clock effect

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