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  • 李文凤,单红丽,王晓燕,陆鑫,张荣跃,仓晓燕,尹炯,罗志明,黄应昆.滇蔗茅杂交F1双抗SCSMV和SrMV鉴定与评价[J].植物遗传资源学报,2017,18(5):886-890.    [点击复制]
  • LI Wen-feng,SHAN Hong-li,WANG Xiao-yan,LU Xin,ZHANG Rong-yue,CANG Xiao-yan,YIN Jiong,LUO Zhi-ming,HUANG Ying-kun.Identification and evaluation of elite sugarcane innovation germplasm resistant to SCSMV and SrMV[J].植物遗传资源学报,2017,18(5):886-890.   [点击复制]
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滇蔗茅杂交F1双抗SCSMV和SrMV鉴定与评价
李文凤,单红丽,王晓燕,陆鑫,张荣跃,仓晓燕,尹炯,罗志明,黄应昆
0
(云南省农业科学院甘蔗研究所)
摘要:
本研究以我国蔗区甘蔗花叶病的2种主要病原甘蔗条纹花叶病毒分离物(SCSMV-JP1,GenBank登录号JF488064)和高粱花叶病毒分离物(SrMV-HH,GenBank登录号DQ530434)为接种毒源,采用人工切茎接种和RT-PCR检测相结合方法,于2015~2016年2次对由热带种‘路打士’与滇蔗茅‘云滇95-19’杂交获得的41份优良创新种质及亲本进行了双抗SCSMV和SrMV鉴定与评价。结果表明,41份优良创新种质及亲本中,对SCSMV表现 1级高抗到3级中抗的有23份,占53.49%,4级感病到5级高感的有20份,占46.51%;对SrMV 表现1级高抗到3级中抗的有31份,占72.09%,4级感病到5级高感的有12份,占27.91%。综合分析结果显示,‘云09-603’、‘云09-604’、‘云09-607’、‘云09-608’、‘云09-619’、‘云09-622’、‘云09-633’、‘云09-635’、‘云09-656’、‘云滇95-19’等10份种质对SCSMV和SrMV均表现1-2级抗病,占23.26%,其中‘云09-604’、‘云09-607’、‘云09-619’、‘云09-633’、‘云09-656’、‘云滇95-19’等6份种质对2种病毒均表现为1级高抗,占13.95%。研究结果明确了41份优良创新种质及亲本对甘蔗花叶病2种主要致病病原的抗性,筛选出双抗SCSMV和SrMV的优良创新种质10份,为深入开展抗甘蔗花叶病育种提供了优良抗源种质和参考依据。
关键词:  甘蔗  优良创新种质  甘蔗条纹花叶病毒(Sugarcane streak mosaic virus, SCSMV)  高粱花叶病毒(Sorghum mosaic virus, SrMV)  抗病性鉴定
DOI:10.13430/j.cnki.jpgr.2017.05.010
投稿时间:2017-01-09修订日期:2017-06-29
基金项目:现代农业产业技术体系建设专项资金资助(CARS-20-2-2);云南省现代农业产业技术体系建设专项资金资助(YNGZTX-4-92)
Identification and evaluation of elite sugarcane innovation germplasm resistant to SCSMV and SrMV
LI Wen-feng,SHAN Hong-li,WANG Xiao-yan,LU Xin,ZHANG Rong-yue,CANG Xiao-yan,YIN Jiong,LUO Zhi-ming,HUANG Ying-kun
(Sugarcane Research Institute,Yunnan Province Academy of Agricultural Science,Yunnan Key Laboratory of Sugarcane Genetic Improvement)
Abstract:
In 2015 and 2016, the two main pathogenic sugarcane streak mosaic virus (SCSMV-JP1, GenBank accession number JF488064) and Sorghum mosaic virus (SrMV-HH, GenBank accession number DQ530434) were used as inoculums, and the double resistance to SCSMV and SrMV was identified and evaluated in 41 elite sugarcane innovative germplasm and parent from the hybrid F1 progenies of saccharum officinarum “Ludashi”and Erianthus rockii “Yundian95-19”by dropping inoculation on stem-section and RT-PCR detection. The results showed that of 41 elite sugarcane innovative germplasm and parent, 23 (53.49%) were highly (Grade 1) to moderately resistant (Grade 3) and 20 (46.51%) were susceptible (Grade 4) to highly susceptible (Grade 5) to SCSMV; 31 (72.09%) were highly (Grade 1) to moderately resistant (Grade 3) and 12 (27.91%) were susceptible (Grade 4) to highly susceptible (Grade 5) to SrMV. Ten germplasm (23.26%) were highly resistant (Grade 1) to resistant (Grade 2) to SCSMV and SrMV, which included Yun09-603, Yun09-604, Yun09-607, Yun09-608, Yun09-619, Yun09-622, Yun09-633, Yun09-635, Yun09-656, Yundian95-19 of 43 elite sugarcane innovative germplasm. Of these, six germplasm (Yun09-604, Yun09-607, Yun09-619, Yun09-633, Yun09-656, Yundian95-19) were the highly resistant (Grade 1) to SCSMV and SrMV, accounting for 13.95%. The results in the present study have determined the resistance of 41 elite sugarcane innovative germplasm and parent to the two main pathogen of mosaic disease, screened the 10 germplasm resistant to SCSMV and SrMV, provide scientific basis and valuable resistance resource for the further breeding of sugarcane cultivars against mosaic disease.
Key words:  Sugarcane  elite innovative germplasm  sugarcane streak mosaic virus  Sorghum mosaic virus  resistance identification

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